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通过用齐整小核菌对植物组织进行生物测定来评估花生杀菌剂的内吸性。

Assessing Systemicity of Peanut Fungicides Through Bioassay of Plant Tissues with Sclerotium rolfsii.

作者信息

Augusto J, Brenneman T B

机构信息

Department of Plant Pathology, University of Georgia Coastal Plain Experiment Station, Tifton 31793.

出版信息

Plant Dis. 2012 Mar;96(3):330-337. doi: 10.1094/PDIS-04-11-0303.

DOI:10.1094/PDIS-04-11-0303
PMID:30727121
Abstract

To better understand movement of systemic fungicides in peanut (Arachis hypogaea), three terminal, fully expanded leaves of primary lateral branches of 'Tifrunner' peanut were treated with prothioconazole + tebuconazole (Provost, 0.29 kg a.i./ha), azoxystrobin (Abound, 0.31 kg a.i./ha), or flutolanil (Moncut, 0.79 kg a.i./ha) in field experiments. Basipetal leaves and pods on the same branch with the treated leaves were sequentially numbered from 1 to 3, with 1 being closest to treated foliage. These nontreated tissues, with newly formed terminal leaves, were sampled 4, 8, and 12 days after treatment for bioassay with Sclerotium rolfsii. All fungicides protected new acropetal leaves while prothioconazole + tebuconazole also provided some inhibition of S. rolfsii in nontreated basipetal leaves but no fungicide protected pods. In the greenhouse, applications of prothioconazole + tebuconazole or prothioconazole (Proline, 0.18 kg a.i./ha) to main stems of 'Georgia Green' provided some protection to leaves from nontreated cotyledonary branches sampled 14 days after last treatment but S. rolfsii was not inhibited on nontreated roots, stems, or pods. The results demonstrate acropetal protection by all fungicides evaluated, and indicate that prothioconazole + tebuconazole or prothioconazole applied to foliage can sometimes reduce diseases in the lower, nontreated portions of the plant.

摘要

为了更好地了解系统性杀菌剂在花生(落花生)中的移动情况,在田间试验中,对‘Tifrunner’花生主侧枝的三片顶生、完全展开的叶片用丙硫菌唑 + 戊唑醇(普洛施,0.29千克有效成分/公顷)、嘧菌酯(阿砣,0.31千克有效成分/公顷)或氟酰胺(割地跑,0.79千克有效成分/公顷)进行处理。对与处理叶片在同一枝条上的向基叶片和荚果依次编号为1至3,1号最靠近处理的叶片。在处理后4天、8天和12天对这些未处理的组织以及新形成的顶生叶片进行采样,用于对罗氏白绢病菌进行生物测定。所有杀菌剂都能保护新的向顶叶片,而丙硫菌唑 + 戊唑醇还能对未处理的向基叶片中的罗氏白绢病菌产生一定抑制作用,但没有杀菌剂能保护荚果。在温室中,对‘Georgia Green’的主茎施用丙硫菌唑 + 戊唑醇或丙硫菌唑(扑力旺,0.18千克有效成分/公顷),在最后一次处理14天后,对来自未处理子叶分支的叶片有一定保护作用,但对未处理的根、茎或荚果上的罗氏白绢病菌没有抑制作用。结果表明,所评估的所有杀菌剂都具有向顶保护作用,并且表明施用于叶片的丙硫菌唑 + 戊唑醇或丙硫菌唑有时可以减少植株下部未处理部分的病害。

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