Sevón N, Dräger B, Hiltunen R, Oksman-Caldentey K -M
Division of Pharmacognosy, Department of Pharmacy, University of Helsinki, (Viikinkaari 5), PO Box 56, FIN-00014, Helsinki, Finland.
Institut für Pharmazeutische Biologie der Martin-Luther-Universität, Hoher Weg 7, D-06108, Halle, Germany.
Plant Cell Rep. 1997 Jun;16(9):605-611. doi: 10.1007/BF01275500.
Mature plants were regenerated via protoplasts fromAgrobacterium rhizogenes-transformed root cultures ofHyoscyamus muticus L., and chemical analyses were performed on 34 individual plants. The regenerated plants showed strong phenotypic differences from clone to clone as well as from the control plants. Polymerase chain reaction studies revealed that the plants exhibiting the strongest phenotypic alterations contained therol (A, B and C) genes, whereas the plants with fewer alterations had lost them. The plants produced hyoscyamine, scopolamine and a range of different calystegins, and considerable somaclonal variation was observed. Alkaloid production in the plants transgenic for therol genes was clearly reduced. The pattern of calystegins was similar within all the regenerated plants lackingrol genes. Among the plants withrol genes, the calystegin B was not detectable. It seems clear that the presence ofrol genes is detrimental to the alkaloid accumulation in the transgenic plants in contrast to hairy root cultures.
通过发根农杆菌转化的天仙子根培养物的原生质体再生出成熟植株，并对34株单株植物进行了化学分析。再生植株在克隆间以及与对照植株之间表现出强烈的表型差异。聚合酶链反应研究表明，表现出最强表型改变的植株含有rol（A、B和C）基因，而改变较少的植株则失去了这些基因。这些植株产生了莨菪碱、东莨菪碱和一系列不同的旋花茄苷，并且观察到了相当大的体细胞克隆变异。转rol基因植株中的生物碱产量明显降低。在所有缺乏rol基因的再生植株中，旋花茄苷的模式相似。在具有rol基因的植株中，未检测到旋花茄苷B。与毛状根培养物相比，rol基因的存在似乎明显不利于转基因植物中生物碱的积累。
