Bose Rahul, Sengupta Mainak, Basu Debabrata, Jha Sumita
Department of Genetics University of Calcutta Kolkata West Bengal India.
Division of Plant Biology Bose Institute Kolkata West Bengal India.
Plant Direct. 2022 Jun 18;6(6):e414. doi: 10.1002/pld3.414. eCollection 2022 Jun.
root oncogenic locus B () is known to induce hairy roots along with triggering several physiological and morphological changes when present as a transgene. However, it is still unknown how this gene triggers these changes within the plant system. In this study, the effect of , when present as a transgene, was assessed on the gene expression levels of auxin response factors (s)-transcription factors which are key players in auxin-mediated responses. The goal was to uncover Auxin/-driven transcriptional networks potentially active and working selectively, if any, in transgenic background, which might potentially be associated with hairy root development. Hence, the approach involved establishing -transgenic plants, selecting s (Nts) for context-relevance using bioinformatics followed by gene expression profiling. It was observed that out of the chosen Nts, Nt and Nt exhibited a consistent pattern of gene upregulation across organ types. In order to understand the significance of these selective gene upregulation, ontology-based transcriptional network maps of the differentially and nondifferentially expressed s were constructed, guided by co-expression databases. The network maps suggested that Nt-Nt might have major deterministic, underappreciated roles to play in root development in a -transgenic background-as observed by higher number of "root-related" biological processes present as nodes compared to network maps for similarly constructed other non-differentially expressed s. Based on the inferences drawn, it is hypothesized that , when present as a transgene, might drive hairy root development by selective induction of Nt and Nt, suggesting a functional link between the two, leading to the specialized and characteristic -associated traits.
已知根致癌位点B（）作为转基因存在时会诱导毛状根形成，并引发多种生理和形态变化。然而，该基因如何在植物系统中触发这些变化仍不清楚。在本研究中，评估了作为转基因存在时对生长素反应因子（s）转录因子基因表达水平的影响，这些转录因子是生长素介导反应的关键参与者。目的是揭示在转基因背景下可能有潜在活性且选择性起作用的生长素/驱动的转录网络，如果有的话，这可能与毛状根发育有关。因此，该方法包括建立转基因植物，使用生物信息学选择与背景相关的s（Nts），然后进行基因表达谱分析。观察到在所选择的Nts中，Nt和Nt在不同器官类型中呈现出一致的基因上调模式。为了理解这些选择性基因上调的意义，在共表达数据库的指导下，构建了差异表达和非差异表达s的基于本体的转录网络图。网络图表明，与类似构建的其他非差异表达s的网络图相比，Nt - Nt可能在转基因背景下的根发育中具有主要的决定性但未被充分认识的作用，这可通过作为节点存在的“根相关”生物学过程数量更多来观察到。基于得出的推论，推测作为转基因存在时，可能通过选择性诱导Nt和Nt来驱动毛状根发育，这表明两者之间存在功能联系，从而导致与相关的特殊和特征性性状。
