Stark-Lorenzen P, Nelke B, Hänßler G, Mühlbach H P, Thomzik J E
Max-Planck-Institut für Züchtungsforschung, D-50829 Köln, Germany, , , , , , DE.
Agrochemical Division, Research/Biotechnology, Bayer AG, D-51368 Leverkusen-Bayerwerk, Germany, , , , , , DE.
Plant Cell Rep. 1997 Jul;16(10):668-673. doi: 10.1007/s002990050299.
A gene derived from grapevine (Vitis vinifera) coding for stilbene synthase has been transferred into protoplasts of the commercially important japonica rice cultivar Nipponbare using PEG-mediated direct gene transfer. Transgenic plants were regenerated from calli selected on kanamycin. Southern blot analysis of genomic DNA isolated from regenerants and progeny plants demonstrated that the stilbene synthase gene is stably integrated in the genome of transgenic rice plants and inherited in the offspring. The transient formation of stilbene-synthase-specific mRNA shortly after inoculation with the fungus of the rice blast Pyricularia oryzae has demonstrated that the grapevine stilbene synthase promoter is also active in monocotyledonous plants. Preliminary results indicate an enhanced resistance of transgenic rice to P. oryzae.
一种编码芪合酶的葡萄（葡萄属）基因已通过聚乙二醇介导的直接基因转移法转入重要的商业粳稻品种日本晴的原生质体中。从在卡那霉素上筛选出的愈伤组织中再生出了转基因植株。对从再生植株和子代植株中分离出的基因组DNA进行的Southern杂交分析表明，芪合酶基因稳定整合到了转基因水稻植株的基因组中，并在后代中得以遗传。在用稻瘟病菌接种后不久芪合酶特异性mRNA的瞬时形成表明，葡萄芪合酶启动子在单子叶植物中也具有活性。初步结果表明转基因水稻对稻瘟病菌的抗性增强。
