Szankowski I, Briviba K, Fleschhut J, Schönherr J, Jacobsen H-J, Kiesecker H
Institut für Gemüse- und Obstbau, Universität Hannover, Am Steinberg 3, 31157 Sarstedt, Germany.
Plant Cell Rep. 2003 Sep;22(2):141-9. doi: 10.1007/s00299-003-0668-8. Epub 2003 Jul 9.
The objective of the present research was to introduce genes with antifungal potential into the commercially important apple cvs. Elstar and Holsteiner Cox in order to establish resistance against fungal diseases. The gene encoding the stilbene synthase (Vst1) from Vitis vinifera L., responsible for the synthesis of the phytoalexin resveratrol in grapevine, and the gene for a polygalacturonase-inhibiting protein (PGIP) from kiwi ( Actinidia deliciosa) were transferred into Holsteiner Cox and Elstar via Agrobacterium tumefaciens-mediated transformation. A total of nine transgenic Holsteiner Cox clones and one transgenic E clone carrying the stilbene-synthase gene as well as three transgenic Holsteiner Cox lines harbouring the polygalacturonase-inhibiting protein from Kiwi were identified via polymerase chain reaction and Southern blot analysis. High performance liquid chromatography analysis revealed the accumulation of a resveratrol-derivate, a glycoside, in transgenic Vst1 plants.
本研究的目的是将具有抗真菌潜力的基因导入具有商业重要性的苹果品种“埃尔斯特”和“霍尔斯泰纳考克斯”，以建立对真菌病害的抗性。编码来自葡萄（Vitis vinifera L.）的芪合酶（Vst1）的基因，该基因负责葡萄中植保素白藜芦醇的合成，以及来自猕猴桃（美味猕猴桃）的多聚半乳糖醛酸酶抑制蛋白（PGIP）基因，通过根癌农杆菌介导的转化被导入“霍尔斯泰纳考克斯”和“埃尔斯特”。通过聚合酶链反应和Southern印迹分析，共鉴定出9个携带芪合酶基因的转基因“霍尔斯泰纳考克斯”克隆和1个转基因E克隆，以及3个含有来自猕猴桃的多聚半乳糖醛酸酶抑制蛋白的转基因“霍尔斯泰纳考克斯”株系。高效液相色谱分析表明，转基因Vst1植株中积累了一种白藜芦醇衍生物——糖苷。
