Teng W -L
Hong Kong Institute of Biotechnology, 2 Biotechnology Avenue, 12 Miles, Tai Po Road, Shatin, N.T., Hong Kong.
Plant Cell Rep. 1997 Apr;16(7):454-457. doi: 10.1007/BF01092765.
A micropropagation scheme forAnanas comosus Merr. was developed using nodule culture. Nodules were induced from leaf-base or chopped shoot-base explants on modified half-strength MS medium supplemented with 2.69-5.37 μM NAA and 4.44 μM BA and could be maintained long-term as nodules. The nodules proliferated into more nodules when chopped into pieces of 1-3 mm and placed onto the same medium. They regenerated shoots when transferred to medium supplemented with 0.54-10.74 μM NAA and 0.44-8.88 μM BA. The regeneration capacity of nodules is higher than that of direct regeneration or callus. Maximum regeneration was obtained from culture medium containing 0.54 μM NAA and 0.44 μM BA, where shoots could be observed as early as within 2 weeks. Many shoots formed roots in the same medium in which they were regenerated after 10 subcultures, but the best rooting occurred in medium containing 0.54 μM NAA and 0.44 μM BA. Rooted plantlets ofA. comosus Merr. could be routinely produced at 6-week intervals.
利用根瘤培养开发了一种用于菠萝（Ananas comosus Merr.）的微繁殖方案。在添加了2.69 - 5.37 μM萘乙酸（NAA）和4.44 μM苄氨基腺嘌呤（BA）的改良半强度MS培养基上，从叶基部或切碎的茎基部外植体诱导出根瘤，并可作为根瘤长期保存。将根瘤切成1 - 3毫米的小块并置于相同培养基上时，它们会增殖形成更多根瘤。当转移到添加了0.54 - 10.74 μM NAA和0.44 - 8.88 μM BA的培养基上时，它们会再生出芽。根瘤的再生能力高于直接再生或愈伤组织。从含有0.54 μM NAA和0.44 μM BA的培养基中获得了最大再生效果，最早在2周内就能观察到芽。许多芽在经过10次继代培养后在其再生的相同培养基中形成根，但最佳生根情况出现在含有0.54 μM NAA和0.44 μM BA的培养基中。菠萝（Ananas comosus Merr.）的生根苗可以每隔6周常规生产一次。
