Department of Botany/Date Palm Research Institute, Shah Abdul Latif University, Khairpur, Sindh, Pakistan.
Tropical Fruit Department, Horticulture Research Institute, Agricultural Research Center, Cairo, Egypt.
Sci Rep. 2023 Jan 23;13(1):1275. doi: 10.1038/s41598-023-28359-9.
A comprehensive study was carried out on in vitro multiplication and rooting using the medium enriched with different plant growth regulators and acclimatization of pineapple cv. 'Smooth Cayenne' using different soil growing substrates. The significantly highest shoot buds (Avg. 16.7) were obtained on the medium comprising 2.0 mg L BA (6-Benzylaminopurine). Results showed that 1.0 mg L IBA (Indole-3-butyric acid) increased the thickness and length of white adventitious roots and resulted in a significantly highest number of roots (Avg. 8) and root length (6.15 cm). Plantlets with healthy, multiple roots were transplanted in several soil combinations of river silt, bolhari (yellow sand), and peat moss. However, the significantly highest survival (100%) of plantlets in the greenhouse was obtained on the soil medium containing only peat moss. Furthermore, soil mixtures of bolhari and peat moss (1:1) and river silt alone exhibited 98.9% and 95.1% survivability of plantlets, which was also considered equally significant (at 5% probability level). The in vitro and ex vitro protocols optimized in the current study can be applied commercially for pineapple crop worldwide.
采用不同植物生长调节剂的培养基进行体外增殖和生根以及不同土壤生长基质对菠萝 cv. ' Smooth Cayenne' 的驯化的综合研究。在包含 2.0 mg/L BA（6-苄基氨基嘌呤）的培养基上获得了最高的芽数（平均 16.7）。结果表明，1.0 mg/L IBA（吲哚-3-丁酸）增加了白色不定根的厚度和长度，并导致根的数量（平均 8）和根长（6.15 cm）显著增加。具有健康、多根的组培苗被移栽到几种土壤混合物中，包括河泥、博哈里沙（黄沙）和泥炭藓。然而，在仅含泥炭藓的土壤基质中，温室中组培苗的存活率（100%）显著最高。此外，博哈里沙和泥炭藓（1:1）的土壤混合物以及单独的河泥分别表现出 98.9%和 95.1%的组培苗存活率，这也被认为同样重要（在 5%概率水平上）。本研究优化的离体和离体外方案可在全球范围内应用于商业菠萝作物。
