Litwińczuk Wojciech, Jacek Beata
Department of Plant Physiology and Biotechnology, Institute of Agricultural Sciences, Land Management and Environmental Protection, University of Rzeszów, Ćwiklińskiej 2nd St., 35-601 Rzeszów, Poland.
Institute of Soil Science and Plant Cultivation, State Research Institute in Puławy, St. Czartoryskich 8.
Plants (Basel). 2020 Nov 10;9(11):1533. doi: 10.3390/plants9111533.
The aim of the study was to compare two methods of micropropagation of mulberry: single-node culture ("SNC"), and axillary-branching ("AxB"). The experiments were carried out on in vitro cultures for 6 successive passages. The "AxB" cultures were propagated on modified MS medium (+ 25% Ca and Mg), supplemented with WPM vitamins, sucrose (30 g L), and BA (1.5 mg l). The "SNC" cultures were grown on cytokinin-free 1/2 MS (macro- and micronutrients) medium supplemented with WPM vitamins, IBA (0.05 mg l), and sucrose (15 g l). Both media (pH 5.8) were solidified with agar (7.0 g l). Initiation of in vitro cultures from explants taken from adult trees and young, potted plants was feasible on both media. Cultures were established from about 1 cm long nodal explants. Generally "SNC" cultures formed one well rooted, significantly longer axillary shoot with bigger leaves than "AxB" cultures, which developed significantly more shoots and big callus at the explant base. All shoots collected from "SNC" and "AxB" cultures rooted in vivo in peat mixture and developed into similar plantlets. The single-node method based on application of cytokinin-free medium is a good alternative for the axillary-branching method for micropropagation of mountain mulberry () "Kenmochi".
单节培养（“SNC”）和腋芽分支（“AxB”）。实验在体外培养中连续进行6代。“AxB”培养物在改良的MS培养基（添加25%的钙和镁）上繁殖，添加WPM维生素、蔗糖（30 g/L）和BA（1.5 mg/L）。“SNC”培养物在不含细胞分裂素的1/2 MS（大量和微量营养素）培养基上生长，添加WPM维生素、IBA（0.05 mg/L）和蔗糖（15 g/L）。两种培养基（pH 5.8）均用琼脂（7.0 g/L）固化。从成年树和盆栽幼树上采集的外植体在两种培养基上都能成功启动体外培养。培养物从约1 cm长的节段外植体建立。一般来说，“SNC”培养物形成一个根系良好、明显更长且叶片更大的腋芽，比“AxB”培养物多，“AxB”培养物在外植体基部发育出更多的芽和大的愈伤组织。从“SNC”和“AxB”培养物中采集到的所有芽在泥炭混合物中于体内生根，并发育成相似的幼苗。基于使用不含细胞分裂素培养基进行单节培养的方法是用于山桑（“Kenmochi”）微繁殖的腋芽分支方法的良好替代方法。
