Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA.
Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, MA.
Blood. 2019 Mar 28;133(13):1446-1456. doi: 10.1182/blood-2018-09-835389. Epub 2019 Feb 6.
We now have the potential to undertake detailed analysis of the inner workings of thousands of cancer cells, one cell at a time, through the emergence of a range of techniques that probe the genome, transcriptome, and proteome combined with the development of bioinformatics pipelines that enable their interpretation. This provides an unprecedented opportunity to better understand the heterogeneity of chronic lymphocytic leukemia and how mutations, activation states, and protein expression at the single-cell level have an impact on disease course, response to treatment, and outcomes. Herein, we review the emerging application of these new techniques to chronic lymphocytic leukemia and examine the insights already attained through this transformative technology.
现在，通过一系列技术的出现，我们有可能对数千个癌细胞的内部运作进行详细分析，一次一个细胞。这些技术包括对基因组、转录组和蛋白质组进行探测，并结合生物信息学管道的开发，以实现对它们的解读。这为更好地了解慢性淋巴细胞白血病的异质性以及突变、激活状态和单细胞水平的蛋白质表达如何影响疾病进程、对治疗的反应和结果提供了前所未有的机会。本文综述了这些新技术在慢性淋巴细胞白血病中的应用，并探讨了通过这项变革性技术已经获得的见解。
