Department of Molecular Developmental Biology, Radboud Institute for Molecular Life Sciences, Science Faculty, Radboud University, PO Box 9101, 6500 HB, Nijmegen, The Netherlands.
Department of Molecular Biology, Radboud Institute for Molecular Life Sciences, Science Faculty, Radboud University, PO Box 9101, 6500 HB, Nijmegen, The Netherlands.
Sci Rep. 2019 Feb 6;9(1):1469. doi: 10.1038/s41598-018-37977-7.
Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogeneous cellular responses to environmental signals at the mRNA and phospho-proteome level.
环境刺激通常会导致异质细胞反应和转录输出。我们开发了单细胞 RNA 和免疫检测（RAID）技术，可从固定的单细胞中同时分析转录组和细胞内（磷酸化）蛋白。RAID 成功地在人类角质形成细胞中重现了蛋白质和 mRNA 水平的分化状态变化。此外，我们还表明，保留高磷酸化 FAK 水平（与干细胞相关的特征）的分化角质形成细胞也表达了一系列与干细胞相关的转录本。我们的数据表明，RAID 允许在 mRNA 和磷酸化蛋白质组水平上研究环境信号对异质细胞反应的影响。
