Key Laboratory for Food Microbial Technology of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xue Zheng Street, Hangzhou 310018, China.
China National Institute of Standardization, No. 4 Zhichun Road, Haidian District, Beijing 100191, China.
Food Res Int. 2018 May;107:1-9. doi: 10.1016/j.foodres.2018.01.067. Epub 2018 Jan 31.
Large yellow croaker (Pseudosciaena crocea) is a popular and nutritious but also highly perishable fish species, with Shewanella baltica being the primary spoilage bacteria during low-temperature storage. Clarifying the factors promoting spoilage will facilitate efforts to predict and control the shelf life of foods. This study focused on spoilage-related genes in two Shewanella baltica strains with different spoilage potentials. Using whole genome sequencing and alignment, three distinguishing genes (torT, cysM and trxB) were identified. Further protein sequence comparison and protein structure modeling revealed possible motifs responsible for the spoilage activity. Moreover, diketopiperazine (DKP) quorum sensing (QS) signaling molecules regulated biofilm formation and spoilage gene expression, indicating a relationship between the QS system, biofilm formation and spoilage potential. Our results suggest that DKPs and spoilage genes are potential targets for developing novel food antiseptics, as well as new markers for fish product spoilage.
大黄鱼（Pseudosciaena crocea）是一种受欢迎且营养丰富的鱼类，但也极易腐坏，在低温贮藏过程中，伯氏假交替单胞菌是主要的致腐细菌。阐明促进腐败的因素将有助于预测和控制食品的保质期。本研究关注了具有不同腐败潜力的两种伯氏假交替单胞菌菌株中的腐败相关基因。通过全基因组测序和比对，鉴定出了三个有区别的基因（torT、cysM 和 trxB）。进一步的蛋白质序列比较和蛋白质结构建模揭示了可能与腐败活性相关的基序。此外，二酮哌嗪（DKP）群体感应（QS）信号分子调节生物膜形成和腐败基因表达，表明 QS 系统、生物膜形成和腐败潜力之间存在关系。我们的研究结果表明，DKPs 和腐败基因可能是开发新型食品防腐剂的潜在目标，也是鱼类产品腐败的新标志物。
