Zhao Yuan, Zheng Hong-Xia, Xu Ying, Lin Na
Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Evid Based Complement Alternat Med. 2019 Jan 8;2019:5493714. doi: 10.1155/2019/5493714. eCollection 2019.
A Chinese herbal preparation, QingYan formula (QYF), has been used clinically for kidney-invigorating. However, no evidence base links QYF to estrogen replacement therapy. In this study, the estrogenic effects of QingYan formula 70% ethanol extract (QYFE) were investigated in immature mice. Immature mice were treated with QYFE at doses of 1, 2, and 4g/kg for 7 days. QYFE treatments promoted vaginal cornification and prolonged the estrus status of the immature mice, promoted the growth and development of uterus and vagina, upregulated ER and ER expression at protein level in uterus and vagina, increased the level of estradiol (E), and decreased concentration of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in serum. This study demonstrated that QYFE exerts estrogenic effects by stimulating biosynthesis of estrogen and increasing estrogen receptors (ERs) in target tissues and provided an evidence base for QYFE treatment instead of estrogen replacement therapy.
一种中药制剂清炎方（QYF）已在临床上用于补肾。然而，尚无证据表明清炎方与雌激素替代疗法有关。在本研究中，对清炎方70%乙醇提取物（QYFE）在未成熟小鼠中的雌激素样作用进行了研究。未成熟小鼠分别用1、2和4g/kg剂量的QYFE处理7天。QYFE处理促进了未成熟小鼠的阴道角化并延长了发情状态，促进了子宫和阴道的生长发育，上调了子宫和阴道中雌激素受体（ER）和ERβ的蛋白表达水平，增加了雌二醇（E2）水平，并降低了血清中促黄体生成素（LH）和促卵泡生成素（FSH）的浓度。本研究表明，QYFE通过刺激雌激素的生物合成和增加靶组织中的雌激素受体（ERs）发挥雌激素样作用，并为QYFE替代雌激素替代疗法提供了证据基础。
