Ganguly Ram Kumar, Chakraborty Susanta Kumar
Department of Zoology, Vidyasagar University, Midnapore, West Bengal 721102 India.
J Environ Health Sci Eng. 2018 Aug 7;16(2):205-212. doi: 10.1007/s40201-018-0308-4. eCollection 2018 Dec.
Main thrust of the present study is to determine the role of microbes in changing the proportion and turnover of nutrients such as carbon and nitrogen during vermicomposting of paper mill sludge through the assessment of β-glucosidase and Leucine arylamidase activities.
The change in the ratio of Total Organic Carbon (TOC) and Total Kjeldahl Nitrogen (TKN) during sludge composting using paper mill sludge, cow dung, straw in the ratio of 5:4:1 have been determined alongside observing β-glucosidase and Leucine arylamidase activities in different phases (0th, 30th and 60th days) of vermicomposting. The present study also assessed the bacterial assemblages in order to predict their role as major producers of these enzymes as estimated by Vitek 2 system.
A declining trend of C/N ratio was observed which exhibited significant negative and positive correlations with the activity of β-glucosidase and Leucine arylamidase respectively. Twenty-four strains were isolated under two genus spp. and spp. which revealed differential sensitivity towards major carbon and nitrogen turnover mediating enzymes.
Change in the activities of carbon and nitrogen turnover enzymes due to microbial growth and proliferation are the mediator for the change in C/N ratio.
本研究的主要目的是通过评估β-葡萄糖苷酶和亮氨酸芳基酰胺酶的活性，确定微生物在造纸厂污泥蚯蚓堆肥过程中改变碳和氮等养分比例及周转中的作用。
测定了以5:4:1的比例使用造纸厂污泥、牛粪、秸秆进行污泥堆肥期间总有机碳（TOC）与总凯氏氮（TKN）的比例变化，同时观察了蚯蚓堆肥不同阶段（第0天、第30天和第60天）的β-葡萄糖苷酶和亮氨酸芳基酰胺酶活性。本研究还评估了细菌群落，以便预测它们作为这些酶的主要生产者的作用，这是通过Vitek 2系统估计的。
观察到碳氮比呈下降趋势，分别与β-葡萄糖苷酶和亮氨酸芳基酰胺酶的活性呈显著负相关和正相关。在两个属和 spp.下分离出24株菌株，它们对主要碳和氮周转介导酶表现出不同的敏感性。
由于微生物生长和增殖导致的碳和氮周转酶活性变化是碳氮比变化的介导因素。
