Wang Xiaobei, Zeng Wenfang, Ding Yifeng, Wang Yan, Niu Liang, Yao Jia-Long, Pan Lei, Lu Zhenhua, Cui Guochao, Li Guohuai, Wang Zhiqiang
1Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, 450009 Zhengzhou, China.
2Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, 430070 Wuhan, China.
Hortic Res. 2019 Feb 1;6:19. doi: 10.1038/s41438-018-0094-2. eCollection 2019.
The plant hormone ethylene regulates ripening in climacteric fruits. The phytohormone abscisic acid (ABA) affects ethylene biosynthesis, but whether ethylene influences ABA biosynthesis is unknown. To explore this possibility, we investigated the interactions between the ABA biosynthesis genes and the ethylene response transcription factor PpERF3 in peach fruit. The ABA content increased during fruit maturation and reached a peak at stage S4 III. The increase was greatly inhibited by the ethylene inhibitor 1-MCP, which also suppressed expression. shared a similar expression profile with , encoding a rate-limiting enzyme involved in ABA biosynthesis, during fruit ripening. A yeast one-hybrid assay suggested that the nuclear-localized PpERF3 might bind to the promoters of . PpERF3 increased the expression of as shown by dual-luciferase reporters, promoter-GUS assays and transient expression analyses in peach fruit. Collectively, these results suggest that ethylene promotes ABA biosynthesis through PpERF3's regulation of the expression of ABA biosynthesis genes .
植物激素乙烯调控跃变型果实的成熟过程。植物激素脱落酸（ABA）影响乙烯生物合成，但乙烯是否影响ABA生物合成尚不清楚。为探究这种可能性，我们研究了桃果实中ABA生物合成基因与乙烯响应转录因子PpERF3之间的相互作用。果实成熟期间ABA含量增加，并在S4 III阶段达到峰值。乙烯抑制剂1-MCP极大地抑制了这种增加，同时也抑制了相关基因的表达。在果实成熟过程中，[相关基因名称]与[另一相关基因名称]具有相似的表达模式，[另一相关基因名称]编码参与ABA生物合成的限速酶。酵母单杂交试验表明，定位于细胞核的PpERF3可能与[相关基因名称]的启动子结合。双荧光素酶报告基因、启动子-GUS分析以及桃果实中的瞬时表达分析表明，PpERF3可增加[相关基因名称]的表达。总体而言，这些结果表明乙烯通过PpERF3对ABA生物合成基因[相关基因名称]表达的调控促进ABA生物合成。
