一株雌激素降解菌SJTE-1的分离与鉴定
Isolation and characterization of an estrogen-degrading strain SJTE-1.
作者信息
Wang Pingping, Zheng Daning, Liang Rubing
机构信息
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240 China.
出版信息
3 Biotech. 2019 Feb;9(2):61. doi: 10.1007/s13205-018-1537-z. Epub 2019 Jan 31.
Abstract
In this report, SJTE1 isolated from an enrichment culture of sludge was confirmed to degrade natural estrogens (17β-estradiol, estrone, estriol), estrogenic chemicals (naphthalene and phenanthrene) and testosterone. The strain completely degraded 1 mg/L 17β-estradiol in 24 h and transformed it into estrone; 90% and 75% of 50 mg/L and 100 mg/L 17β-estradiol were utilized in 7 days, respectively. The transformation efficiency of this strain against natural estrogens was much higher than that against other estrogenic chemicals. Organic carbon sources, lipopolysaccharide and surfactants could enhance the degradation efficiency of strain SJTE-1 against 17β-estradiol. The adsorption of 17β-estradiol onto the biomass was the premise for transmembrane and cellular utilization of this chemical. This work has the potential to bioremediate the environmental estrogens.
摘要
在本报告中，从污泥富集培养物中分离出的SJTE1被证实可降解天然雌激素（17β-雌二醇、雌酮、雌三醇）、雌激素类化学物质（萘和菲）以及睾酮。该菌株在24小时内完全降解了1毫克/升的17β-雌二醇，并将其转化为雌酮；50毫克/升和100毫克/升的17β-雌二醇分别在7天内被利用了90%和75%。该菌株对天然雌激素的转化效率远高于对其他雌激素类化学物质的转化效率。有机碳源、脂多糖和表面活性剂可提高菌株SJTE-1对17β-雌二醇的降解效率。17β-雌二醇在生物质上的吸附是该化学物质跨膜和细胞利用的前提。这项工作具有对环境雌激素进行生物修复的潜力。
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