Adiguzel Mehmet Cemal, Sigirci Belgi Diren, Celik Baran, Kahraman Beren Basaran, Metiner Kemal, Ikiz Serkan, Bagcigil A Funda, Ak Seyyal, Ozgur N Yakut
Department of Microbiology, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey.
Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University, 34320, Istanbul, Turkey.
J Vet Res. 2018 Dec 31;62(4):463-468. doi: 10.2478/jvetres-2018-0071. eCollection 2018 Dec.
The study aimed to isolate thermophilic from chickens raised three rearing methods, determine its antimicrobial susceptibilities, and examine resistance-related genes by PCR.
Cloacal swabs or intestinal contents were taken in Istanbul, Sakarya, and Izmir provinces. Chickens were from small village-based family-run businesses (n = 70), organically raised (n = 71), and conventionally raised broilers (n = 79). The samples were cultured on modified charcoal cefoperazone desoxycholate (mCCD) agar. Suspect isolates were identified with multiplex PCR (mPCR). As per EUCAST standards, MIC values were derived by broth microdilution for tetracycline, ciprofloxacin, nalidixic acid, kanamycin, gentamicin, and erythromycin in isolates of (n = 98) and (n = 83).
In , 78.6% tetracycline, 87.8% ciprofloxacin, and 81.6% nalidixic acid resistance was detected, but none was to kanamycin, gentamicin, or erythromycin. In , 98.8% ciprofloxacin and 63.9% nalidixic acid resistance was detected, whereas resistance to non-quinolones was not observed. C257T (Thr-86-Ile) mutation in the gene of all phenotypically quinolone-resistant isolates was detected through a mismatch amplification mutation assay PCR (MAMA-PCR). It emerged that all isolates bore the (O) resistance gene.
Common tetracycline, nalidixic acid, and ciprofloxacin resistance exists in isolated from chickens raised three rearing methods.
本研究旨在从采用三种饲养方式饲养的鸡中分离嗜热菌，确定其抗菌药敏性，并通过聚合酶链反应（PCR）检测耐药相关基因。
在伊斯坦布尔、萨卡里亚和伊兹密尔省采集泄殖腔拭子或肠道内容物。鸡来自小型乡村家庭经营企业（n = 70）、有机饲养（n = 71）和传统饲养的肉鸡（n = 79）。样本在改良的木炭头孢哌酮脱氧胆酸盐（mCCD）琼脂上培养。可疑分离株通过多重聚合酶链反应（mPCR）进行鉴定。根据欧洲抗菌药物敏感性试验委员会（EUCAST）标准，通过肉汤微量稀释法测定98株和83株分离株中四环素、环丙沙星、萘啶酸、卡那霉素、庆大霉素和红霉素的最低抑菌浓度（MIC）值。
在……中，检测到78.6%的四环素耐药、87.8%的环丙沙星耐药和81.6%的萘啶酸耐药，但对卡那霉素、庆大霉素或红霉素均无耐药。在……中，检测到98.8%的环丙沙星耐药和63.9%的萘啶酸耐药，而未观察到对非喹诺酮类药物的耐药。通过错配扩增突变分析聚合酶链反应（MAMA-PCR）检测到所有表型喹诺酮耐药分离株的gyrA基因中存在C257T（Thr-86-Ile）突变。结果表明，所有分离株均携带blaCTX-M（O）耐药基因。
从采用三种饲养方式饲养的鸡中分离出的……存在常见的四环素、萘啶酸和环丙沙星耐药情况。
