Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708, USA.
Appl Environ Microbiol. 2010 Dec;76(24):7949-56. doi: 10.1128/AEM.01297-10. Epub 2010 Oct 22.
The emergence of antimicrobial resistance in Campylobacter spp. has been a growing public health concern globally. The objectives of this study were to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of Campylobacter spp. recovered by the National Antimicrobial Resistance Monitoring System (NARMS) retail meat program. Retail meat samples (n = 24,566) from 10 U.S. states collected between 2002 and 2007, consisting of 6,138 chicken breast, 6,109 ground turkey, 6,171 ground beef, and 6,148 pork chop samples, were analyzed. A total of 2,258 Campylobacter jejuni, 925 Campylobacter coli, and 7 Campylobacter lari isolates were identified. Chicken breast samples showed the highest contamination rate (49.9%), followed by ground turkey (1.6%), whereas both pork chops and ground beef had <0.5% contamination. The most common resistance was to doxycycline/tetracycline (46.6%), followed by nalidixic acid (18.5%), ciprofloxacin (17.4%), azithromycin and erythromycin (2.8%), telithromycin (2.4%), clindamycin (2.2%), and gentamicin (<0.1%). In a subset of isolates tested, no resistance to meropenem and florfenicol was seen. C. coli isolates showed higher resistance rates to antimicrobials, with the exception of doxycycline/tetracycline, than those seen for C. jejuni. Pulsed-field gel electrophoresis (PFGE) fingerprinting resulted in 1,226 PFGE profiles among the 2,318 isolates, with many clones being widely dispersed throughout the 6-year sampling period.
弯曲菌属中抗菌药物耐药性的出现是一个日益受到全球关注的公共卫生问题。本研究的目的是确定全国抗菌药物耐药性监测系统（NARMS）零售肉类项目中分离的弯曲菌属的流行率、抗菌药物敏感性和遗传相关性。2002 年至 2007 年，从美国 10 个州采集了 24566 份零售肉类样本（包括 6138 份鸡胸肉、6109 份火鸡肉碎、6171 份碎牛肉和 6148 份猪排），对这些样本进行了分析。共鉴定出 2258 株空肠弯曲菌、925 株大肠弯曲菌和 7 株拉里弯曲菌。鸡胸肉样本的污染率最高（49.9%），其次是火鸡肉碎（1.6%），而猪排和碎牛肉的污染率均<0.5%。最常见的耐药性是对强力霉素/四环素（46.6%），其次是萘啶酸（18.5%）、环丙沙星（17.4%）、阿奇霉素和红霉素（2.8%）、替马环素（2.4%）、克林霉素（2.2%）和庆大霉素（<0.1%）。在测试的分离株亚组中，未发现对美罗培南和氟苯尼考的耐药性。与空肠弯曲菌相比，大肠弯曲菌对各种抗菌药物的耐药率更高，除了强力霉素/四环素。脉冲场凝胶电泳（PFGE）指纹图谱分析显示，在 2318 株分离株中，有 1226 种 PFGE 图谱，许多克隆在 6 年的采样期间广泛分布。
