Salih Erdjan
Department of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University Medical Center, Boston, MA, USA.
Methods Mol Biol. 2019;1914:217-240. doi: 10.1007/978-1-4939-8997-3_11.
This chapter elaborates on the state-of-the-art experimental procedures utilized in ex-vivo model systems of cancer-bone cell interactions under "static and dynamic" culture conditions and their potential use to understand cellular and molecular mechanisms as well as drug testing and discovery. An additional focus of this chapter is to provide details of how to incorporate varying oxygen tension, viz., hypoxic, normoxic, and hyperoxic, in such studies and regulate the bone biology toward dissociation of the bone remodeling stages to achieve only "bone resorption" or "bone formation" individually.
本章详细阐述了在“静态和动态”培养条件下，用于癌症-骨细胞相互作用的体外模型系统的最新实验程序，以及它们在理解细胞和分子机制以及药物测试与发现方面的潜在用途。本章的另一个重点是提供在这类研究中如何引入不同氧张力，即低氧、常氧和高氧，并调节骨生物学以解离骨重塑阶段，从而单独实现“骨吸收”或“骨形成”的详细信息。
