Staines Katherine A, Brown Genevieve, Farquharson Colin
School of Applied Sciences, Edinburgh Napier University, Edinburgh, UK.
Department of Biomedical Engineering, Columbia University, New York, NY, USA.
Methods Mol Biol. 2019;1914:199-215. doi: 10.1007/978-1-4939-8997-3_10.
The ex vivo organ culture of bone provides many of the advantages of both the whole organism and isolated cell strategies and can deliver valuable insight into the network of processes and activities that are fundamental to bone and cartilage biology. Through maintaining the bone and/or cartilage cells in their native environment, this model system provides the investigator with a powerful experimental protocol to address specific facets of skeletal growth and development. In this chapter, we outline the basic protocols and possible readouts of organ culture models to replicate; (a) linear bone growth (murine metatarsal culture model), (b) bone and cartilage metabolism (murine femoral head culture model), (c) bone response to mechanical stimulation (bovine trabecular core culture model), and (d) bone resorption and formation (murine calvaria culture model).
骨的体外器官培养具有整体生物体和分离细胞策略的诸多优点，能够为深入了解对骨和软骨生物学至关重要的一系列过程和活动网络提供有价值的见解。通过将骨和/或软骨细胞维持在其天然环境中，该模型系统为研究人员提供了一个强大的实验方案，以解决骨骼生长和发育的特定方面。在本章中，我们概述了用于复制以下内容的器官培养模型的基本方案和可能的读数：(a) 线性骨生长（小鼠跖骨培养模型），(b) 骨和软骨代谢（小鼠股骨头培养模型），(c) 骨对机械刺激的反应（牛小梁核心培养模型），以及(d) 骨吸收和形成（小鼠颅骨培养模型）。
