Caetano-Silva Soraia P, Novicky Astrid, Javaheri Behzad, Rawlinson Simon C F, Pitsillides Andrew A
Skeletal Biology Group, Comparative Biomedical Sciences, The Royal Veterinary College, London, UK.
Barts and The London School of Medicine and Dentistry, Institute of Dentistry, Queen Mary University of London, London, UK.
Methods Mol Biol. 2019;1914:99-128. doi: 10.1007/978-1-4939-8997-3_6.
The techniques that are useful for applying mechanical strain to bone and bone cells are now more diverse than described in the second Edition. Their output has also increased substantially and, perhaps most importantly, their significance is now broadly accepted. This growth in the use of methods for applying mechanical strain to bone and its constituent cells and increased awareness of the importance of the mechanical environment in controlling normal bone cell behavior has indeed heralded new therapeutic approaches. We have expanded the text to include additions and modifications made to the straining apparatus and updated the research cited to support this growing role of cell cultures, including co-culture systems and primary cells, tissue engineering, and organ culture models to analyze responses of bone cells to mechanical stimulation. We understand that there are approaches not covered here and appreciate that alternative strategies have their own value and utility.
与第二版所述相比，目前用于对骨骼和骨细胞施加机械应变的技术更加多样。它们的输出也大幅增加，也许最重要的是，其重要性现在已得到广泛认可。用于对骨骼及其组成细胞施加机械应变的方法的使用增加，以及对机械环境在控制正常骨细胞行为中的重要性的认识提高，确实预示着新的治疗方法的出现。我们扩充了文本内容，纳入了对拉伸装置所做的补充和修改，并更新了所引用的研究，以支持细胞培养（包括共培养系统和原代细胞）、组织工程以及器官培养模型在分析骨细胞对机械刺激反应方面日益重要的作用。我们明白这里未涵盖所有方法，也认识到替代策略有其自身的价值和用途。
