骨的透射电子显微镜检查

Transmission Electron Microscopy of Bone.

作者信息

Everts Vincent, Niehof Anneke, Tigchelaar-Gutter Wikky, Beertsen Wouter

机构信息

Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, VU University Amsterdam, Amsterdam, The Netherlands.

Research Institute MOVE, VU University Amsterdam, Amsterdam, The Netherlands.

出版信息

Methods Mol Biol. 2019;1914:617-629. doi: 10.1007/978-1-4939-8997-3_32.

DOI:10.1007/978-1-4939-8997-3_32

PMID:30729488

Abstract

Electron microscopic analysis of mineralized tissues like bone and dentin is essential for understanding of cell-cell/cell-matrix interactions, and the three-dimensional organization of these tissues. This chapter describes a few methods to process mineralized tissues obtained from different sources for ultrastructural analysis by transmission electron microscopy.

摘要

对骨和牙本质等矿化组织进行电子显微镜分析，对于理解细胞间/细胞与基质间的相互作用以及这些组织的三维结构至关重要。本章介绍了几种处理从不同来源获取的矿化组织的方法，以便通过透射电子显微镜进行超微结构分析。