Allocca Gloria, Wang Ning
Department of Oncology and Metabolism, The Mellanby Centre for Bone Research, The University of Sheffield, Sheffield, UK.
Methods Mol Biol. 2019;1914:631-639. doi: 10.1007/978-1-4939-8997-3_33.
Two-photon microscopy has been widely accepted as a powerful tool to provide both qualitative and quantitative information in bone research. This chapter will describe a step-by-step protocol for using two-photon microscopy to track the colonization of cancer cells to bone using frozen bone samples of xenograft mouse models.
双光子显微镜已被广泛认可为一种在骨研究中提供定性和定量信息的强大工具。本章将描述一个逐步的方案，用于使用双光子显微镜，通过异种移植小鼠模型的冷冻骨样本追踪癌细胞在骨中的定植情况。
