Jacques Camille, Renema Nathalie, Ory Benjamin, Walkley Carl R, Grigoriadis Agamemnon E, Heymann Dominique
INSERM, UMR1238, Bone Sarcoma and Remodeling of Calcified Tissues, Université de Nantes, Nantes Atlantique Universités, Nantes, France.
INSERM, U1238, University of Nantes, Nantes, France.
Methods Mol Biol. 2019;1914:331-342. doi: 10.1007/978-1-4939-8997-3_18.
This chapter describes the procedures for inducing bone sarcoma in mice. Two models based on inoculation of cancer cells in paraosseous and intraosseous site will be described. In addition to providing technical aspects of anesthesia and surgical options, key information of cell preparation and postoperative follow-up will be discussed.
本章描述了在小鼠中诱导骨肉瘤的程序。将介绍基于在骨旁和骨内接种癌细胞的两种模型。除了提供麻醉和手术选择的技术方面，还将讨论细胞制备和术后随访的关键信息。
