Department of Chemistry, University of California, Riverside, CA, USA.
Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
J Sci Food Agric. 2019 Jun;99(8):4036-4042. doi: 10.1002/jsfa.9631. Epub 2019 Mar 25.
Pomegranate (Punica granatum L.) - a delicious fruit once used in Ayurvedic medicine - is now largely known for the antioxidant properties of its juice, which has also been considered to have health benefits against diseases such as cancer and cardiovascular diseases. These beneficial effects are associated with the fruit's high content of polyphenolic compounds. High demand and lower production levels drive pomegranate prices up, which leads to the possibility of pomegranate products being adulterated, diluted or substituted. To ensure the presence of pomegranate in various preparations labeled as containing pomegranate, a simple method was developed to screen and quantify the specific punicalagins by mass spectrometry.
The present method was used to analyze several pure and mixed beverages from the US market, and also to quantify punicalagins in the juice of 14 pomegranate cultivars. Punicalagins were detected in all cultivars, with higher concentrations in whole fruit juices compared with aril juices. Amongst the 20 commercial beverages, punicalagins were not detected in four preparations.
The liquid chromatographic-mass spectrometric method presented herein enables an easy and rapid quantification of the specific punicalagins. The latter was detected in all cultivar samples, thus supporting that punicalagin is a suitable marker of these 14 pomegranate cultivars in commercial juices. Absence of the specific marker in four commercial preparations shows the necessity of having simple and rapid methods to evaluate the presence of pomegranate in preparations. © 2019 Society of Chemical Industry.
石榴（Punica granatum L.）——一种曾在印度阿育吠陀医学中使用的美味水果——如今因其果汁的抗氧化特性而广为人知，其果汁也被认为对癌症和心血管疾病等疾病有健康益处。这些有益效果与水果中多酚类化合物的高含量有关。高需求和低产量导致石榴价格上涨，这使得石榴产品有可能被掺假、稀释或替代。为了确保各种标有含有石榴的制剂中存在石榴，开发了一种通过质谱法筛选和定量特定安石榴苷的简单方法。
本方法用于分析来自美国市场的几种纯和混合饮料，以及定量 14 个石榴品种的果汁中的安石榴苷。所有品种均检测到安石榴苷，全果汁中的浓度高于果汁中的浓度。在 20 种商业饮料中，有 4 种制剂未检测到安石榴苷。
本文介绍的液相色谱-质谱法能够轻松快速地定量特定的安石榴苷。在后一种情况下，所有品种样本中均检测到安石榴苷，因此支持安石榴苷是这些 14 个石榴品种在商业果汁中的合适标志物。在四种商业制剂中未检测到特定标志物表明，有必要开发简单快速的方法来评估制剂中石榴的存在。 © 2019 化学工业协会。
