Dipartimento di Sanità Pubblica e Malattie Infettive, Università Sapienza, Rome, Italy.
Centro Agricoltura Ambiente "G.Nicoli", Crevalcore, Italy.
Pest Manag Sci. 2019 Oct;75(10):2642-2651. doi: 10.1002/ps.5369. Epub 2019 Apr 8.
The highly invasive mosquito species Aedes albopictus has become a major health concern in temperate areas due to its role as vector of exotic arboviruses. Pyrethroid insecticides represent the main tools for limiting the circulation of such mosquito-borne viruses. The present work aim to extend previous reports on phenotypic pyrethroid-resistance in European Ae. albopictus, to identify its genetic basis and to monitor the geographical distribution of resistant genotypes, with a particular focus on sites experiencing the 2017 chikungunya outbreak in Italy.
Bioassays, performed according to World Health Organization protocols, showed full susceptibility to deltamethrin (concentration = 0.05%) and varying levels of resistance to permethrin (0.75%) and/or α-cypermethrin (0.05%) across Italy, with highest levels in the core of the 2017 chikungunya outbreak. Partial genotyping of the VSSC gene revealed widespread distribution of V1016G mutation and confirmed its association with pyrethroid resistance.
The results obtained show that the condition for the spread of pyrethroid resistance in Ae. albopictus in Europe exists under strong selective pressure due to intensive insecticide spraying to control exotic arbovirus outbreak or high levels of nuisance. The results draw attention to the need for an evidence-based implementation of mosquito nuisance control, taking insecticide resistance management into consideration. © 2019 Society of Chemical Industry.
高度侵袭性的蚊子物种白纹伊蚊由于其作为外来虫媒病毒载体的作用，已成为温带地区的主要健康关注点。拟除虫菊酯类杀虫剂是限制此类蚊媒病毒传播的主要手段。本研究旨在扩展先前关于欧洲白纹伊蚊表型拟除虫菊酯抗性的报告，确定其遗传基础，并监测具有抗性基因型的地理分布，特别关注 2017 年意大利基孔肯雅热爆发的地点。
根据世界卫生组织的方案进行的生物测定表明，意大利各地对溴氰菊酯（浓度=0.05%）完全敏感，对氯菊酯（0.75%）和/或α-氯氰菊酯（0.05%）的抗性水平不同，2017 年基孔肯雅热爆发的核心地区抗性水平最高。VSSC 基因的部分基因分型显示 V1016G 突变广泛分布，并证实其与拟除虫菊酯抗性有关。
研究结果表明，在欧洲，由于为控制外来虫媒病毒爆发或高度滋扰而进行密集的杀虫剂喷洒，或由于存在强烈的选择压力，白纹伊蚊中拟除虫菊酯抗性传播的条件已经存在。结果提请注意需要在进行蚊虫滋扰控制时，考虑到杀虫剂抗性管理，实施基于证据的策略。 © 2019 英国化学学会。
