a Institute of Nano Science and Technology , Mohali , Punjab , India.
Int J Hyperthermia. 2019;36(1):302-312. doi: 10.1080/02656736.2019.1565787. Epub 2019 Feb 7.
Controlling the magnetic properties of a nanoparticle efficiently via its particle size to achieve optimized heat under alternating magnetic field is the central point for magnetic hyperthermia-mediated cancer therapy (MHCT). Here, we have shown the successful use of stevioside (a natural plant-based glycoside) as a promising biosurfactant to control the magnetic properties of FeO nanoparticles by controlling the particle size. The biocompatibility and cellular uptake efficiency by rat C6 glioma cells and calorimetric magnetic hyperthermia profile of the nanoparticles were further examined. Our finding suggests superior properties of stevioside-coated magnetite nanoparticles in comparison to polysorbate-80 and oleic acid coated nanomagnets as far as particle size reduction, biocompatibility, hyperthermic effect, and cellular uptake by the glioblastoma cancer cells are concerned. The stevioside-coated nanomagnets exhibiting the maximum temperature rise were further investigated as heating agents in in vitro magnetic hyperthermia experiments (405 kHz, 168 Oe), showing their efficacy to induce cell death of rat C6 glioma cells after 30 min at a target temperature T = 43 °C.
通过控制纳米颗粒的粒径来有效控制其磁性，以在交变磁场下达到优化的热量，是磁热疗介导的癌症治疗（MHCT）的核心。在这里，我们成功地使用甜菊糖苷（一种天然植物来源的糖苷）作为一种有前途的生物表面活性剂，通过控制粒径来控制 FeO 纳米颗粒的磁性。进一步研究了纳米颗粒的生物相容性和大鼠 C6 神经胶质瘤细胞的摄取效率以及量热磁热疗曲线。与聚山梨酯 80 和油酸包覆的纳米磁铁相比，我们的发现表明甜菊糖包覆的磁铁纳米颗粒具有优越的性能，就粒径减小、生物相容性、热效应和神经胶质瘤癌细胞的摄取而言。在体外磁热疗实验（405 kHz，168 Oe）中，进一步研究了具有最大温升的甜菊糖包覆纳米磁铁作为加热剂，在目标温度 T=43°C 下 30 分钟后显示出诱导大鼠 C6 神经胶质瘤细胞死亡的功效。
