Santillo Antonella, Figliola Lucia, Caroprese Mariangela, Marino Rosaria, dʼApolito Maria, Giardino Ida, Albenzio Marzia
Department of the Sciences of Agriculture,Food and Environment (SAFE), University of Foggia,Via Napoli, 25, 71122 Foggia,Italy.
Department of Medical and Surgical Sciences,University of Foggia,Viale Pinto, 1, 71122 Foggia,Italy.
J Dairy Res. 2019 Feb;86(1):129-133. doi: 10.1017/S0022029919000104. Epub 2019 Feb 7.
We evaluated the effect of in vitro digested milk on mature adipocytes 3T3-L1, paying particular attention to its fatty acid composition, and comparing human (HM), donkey (DM), bovine (BM), ovine (OM), caprine (CM) and formula (FM) milk. Cellular viability, apoptosis, oxidative response and gene expression levels of NF-κB p65, HMGB1, SREBP-1c and FAS were evaluated. Digested milk treatments significantly reduced 3T3-L1 mature adipocytes viability and caspase activity compared with control group, but no significant differences were observed among different sources of digested milk. In all digested milk samples, ROS level was higher than the control, however, the digested human and formula milk showed lower levels of ROS than DM, BM, OM and CM samples. Lower capacity of HM and FM to induce oxidative stress in mature adipocytes was ascribed to the peculiar free fatty acids profile of digested milk samples. All milk treatments elicited a significant over-expression of NF-κB p65 in 3T3-L1 adipocytes compared to the control; the lowest gene expression was found in HM, BM, OM and CM, the highest in FM and an intermediate behavior was shown in DM. All digested milk treatments influenced the gene expression of SRBP-1c with FM and HM showing the highest levels. For FAS expression, BM showed the highest level, OM and CM intermediate and FM, HM and DM the lowest levels, however HM and DM had comparable levels to the control.
我们评估了体外消化的牛奶对成熟脂肪细胞3T3-L1的影响，特别关注其脂肪酸组成，并比较了人乳（HM）、驴乳（DM）、牛乳（BM）、羊乳（OM）、山羊乳（CM）和配方奶（FM）。评估了细胞活力、凋亡、氧化反应以及NF-κB p65、HMGB1、SREBP-1c和FAS的基因表达水平。与对照组相比，消化牛奶处理显著降低了3T3-L1成熟脂肪细胞的活力和半胱天冬酶活性，但不同来源的消化牛奶之间未观察到显著差异。在所有消化牛奶样品中，活性氧水平均高于对照组，然而，消化后的人乳和配方奶样品的活性氧水平低于驴乳、牛乳、羊乳和山羊乳样品。人乳和配方奶在成熟脂肪细胞中诱导氧化应激的能力较低归因于消化牛奶样品中独特的游离脂肪酸谱。与对照组相比，所有牛奶处理均使3T3-L1脂肪细胞中NF-κB p65显著过表达；人乳、牛乳、羊乳和山羊乳中的基因表达最低，配方奶中最高，驴乳表现出中间水平。所有消化牛奶处理均影响SRBP-1c的基因表达，配方奶和人乳中的水平最高。对于FAS表达，牛乳中的水平最高，羊乳和山羊乳处于中间水平，配方奶、人乳和驴乳中的水平最低，然而人乳和驴乳中的水平与对照组相当。
