Kim Eun Ju, Lee Hyun-Il, Chung Kyung-Jin, Noh Yun-Hee, Ro Youngtae, Koo Ja-Hyun
Department of Biochemistry, School of Medicine, Konkuk University, Seoul 143-701, Korea.
BMB Rep. 2009 Apr 30;42(4):194-9. doi: 10.5483/bmbrep.2009.42.4.194.
The effects of the ginsenoside Rb2 (Rb2) on lipid metabolism were characterized in 3T3-L1 adipocytes to evaluate their utility for treating obesity. While the amounts of total cholesterol and triacylglycerol (TAG) were markedly increased in the adipocytes treated with high amounts of cholesterol and fetal bovine serum (FBS), the test groups treated with Rb2 showed levels that were close to normal. The effect of Rb2 on these cells was comparable to that of lovastatin. Rb2 enhanced the expression of the sterol regulated element binding protein (SREBP) mRNA whereas treatment with cholesterol and FBS led to a reduction in the abundance of this transcript. The activity of fatty acid synthetase (FAS) was lower in the cholesterol group compared to the Rb2 treatment group suggesting that the observed decrease in cholesterol levels and activated SREBP was mediated by Rb2. Treatment with Rb2 also resulted in a decrease in TAG levels in adipocytes cultured under high fatty acid conditions. This effect was mediated by stimulating the expression of SREBP and Leptin mRNA, suggesting that Rb2 might be a valuable component capable of lowering the levels of lipids.
在3T3-L1脂肪细胞中研究了人参皂苷Rb2（Rb2）对脂质代谢的影响，以评估其治疗肥胖症的效用。在用大量胆固醇和胎牛血清（FBS）处理的脂肪细胞中，总胆固醇和三酰甘油（TAG）的含量显著增加，而用Rb2处理的试验组显示的水平接近正常。Rb2对这些细胞的作用与洛伐他汀相当。Rb2增强了固醇调节元件结合蛋白（SREBP）mRNA的表达，而用胆固醇和FBS处理导致该转录本丰度降低。与Rb2处理组相比，胆固醇组中脂肪酸合成酶（FAS）的活性较低，这表明观察到的胆固醇水平降低和SREBP激活是由Rb2介导的。用Rb2处理还导致在高脂肪酸条件下培养的脂肪细胞中TAG水平降低。这种作用是通过刺激SREBP和瘦素mRNA的表达介导的，表明Rb2可能是一种能够降低脂质水平的有价值成分。
