Rosenberg Adrian, Nettey Oluwarotimi S, Gogana Pooja, Sheikh Ujalla, Macias Virgilia, Kajdacsy-Balla Andre, Sharifi Roohollah, Kittles Rick A, Murphy Adam B
Department of Urology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Department of Pathology, University of Illinois at Chicago School of Medicine, Chicago, IL, USA.
Cancer Causes Control. 2019 Feb;30(2):207-214. doi: 10.1007/s10552-019-1128-2. Epub 2019 Feb 7.
To investigate the correlation between serum 25 hydroxyvitamin D, prostatic 25 hydroxyvitamin D, and serum 1,25 dihydroxyvitamin D, and their respective associations with prostatic tumor proliferation at the time of radical prostatectomy.
In this cross-sectional analysis of 119 men undergoing radical prostatectomy, serum from whole blood and expressed prostatic fluid was collected on the day of surgery. Tumor proliferation was measured in the dominant tumor on formalin-fixed prostatectomy tissues by immunohistochemical staining for Ki67 and quantified by Aperio imaging analysis.
The sample included 88 African Americans (74%) and 31 (26%) European Americans. Serum and prostatic levels of 25 hydroxyvitamin D were correlated with each other (Spearman's rho (ρ) = 0.27, p = 0.004), and there was also a correlation between serum 25 hydroxyvitamin D and 1,25 dihydroxyvitamin D (ρ = 0.34, p < 0.001). Serum and prostatic 25 hydroxyvitamin D levels were not correlated with Ki67 staining in tumor cells. Serum 1,25 dihydroxyvitamin D was inversely correlated with Ki67 staining in tumor cells (ρ = - 0.30, p = 0.002). On linear regression, serum 1,25 dihydroxyvitamin D was negatively associated with Ki67 staining in tumor cells (β - 0.46, 95% CI - 0.75, - 0.04, p = 0.04).
The correlation between physiologic serum levels of 25 hydroxyvitamin D with both prostatic 25 hydroxyvitamin D and serum 1,25 dihydroxyvitamin D suggests that serum levels are reasonable biomarkers of vitamin D status. Furthermore, serum 1,25 dihydroxyvitamin D has an inverse association with Ki67 staining in tumor cells at physiologic levels and may protect against tumor progression.
研究血清25羟维生素D、前列腺组织中的25羟维生素D以及血清1,25二羟维生素D之间的相关性，及其与根治性前列腺切除术时前列腺肿瘤增殖的各自关联。
在对119例行根治性前列腺切除术的男性进行的横断面分析中，于手术当天采集全血血清和前列腺液。通过对福尔马林固定的前列腺切除组织中的主要肿瘤进行Ki67免疫组织化学染色来测量肿瘤增殖，并通过Aperio图像分析进行定量。
样本包括88名非裔美国人（74%）和31名（26%）欧裔美国人。血清和前列腺组织中的25羟维生素D水平相互相关（斯皮尔曼等级相关系数（ρ）=0.27，p=0.004），血清25羟维生素D与1,25二羟维生素D之间也存在相关性（ρ=0.34，p<0.001）。血清和前列腺组织中的25羟维生素D水平与肿瘤细胞中的Ki67染色无关。血清1,25二羟维生素D与肿瘤细胞中的Ki67染色呈负相关（ρ=-0.30，p=0.002）。在线性回归分析中，血清1,25二羟维生素D与肿瘤细胞中的Ki67染色呈负相关（β=-0.46，95%置信区间为-0.75，-0.04，p=0.04）。
生理血清水平的25羟维生素D与前列腺组织中的25羟维生素D和血清1,25二羟维生素D之间的相关性表明，血清水平是维生素D状态的合理生物标志物。此外，生理水平的血清1,25二羟维生素D与肿瘤细胞中的Ki67染色呈负相关，可能预防肿瘤进展。
