Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China; Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology, University of Florida, Gainesville, FL, USA.
Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology, University of Florida, Gainesville, FL, USA.
J Proteomics. 2019 Mar 30;196:81-91. doi: 10.1016/j.jprot.2019.02.001. Epub 2019 Feb 5.
Jasmonates (JAs) are important phytohormones that regulate a wide range of plant processes, including growth, development and stress responses. Jasmonate ZIM-domain (JAZ) proteins are transcriptional repressors in JA signaling. Overexpression of JAZ7 was found to confer drought tolerance in Arabidopsis thaliana (A. thaliana), but the molecular mechanisms are not known. Using Tandem Mass Tag (TMT) quantitative proteomics and targeted metabolomics approaches, we found that 394 unique proteins and 96 metabolites were differentially expressed under drought and/or among the three genotypes (wild type (WT), JAZ7 knock out (KO) and JAZ7 overexpression (OE)). Unique and differential proteins/metabolites after each comparison were analyzed to gauge their potential functions in drought tolerance. The proteins and metabolites are enriched in JA and abscisic acid (ABA) signaling pathways, response to stress, photosynthesis, redox and metabolic process. BIOLOGICAL SIGNIFICANCE: Drought stress is a global challenge that affects agricultural production. JAZ7 overexpression led to drought tolerance in A. thaliana through modulating photosynthesis, redox, and amino acids, phytohormones and defense metabolites. The results have provided important insights into the JAZ7 regulated molecular networks of drought tolerance. The knowledge may facilitate effort to enhance crop drought tolerance in the era of climate change.
茉莉酸（JAs）是一类重要的植物激素，调控植物生长、发育和应激响应等多种生理过程。茉莉酸 ZIM 结构域（JAZ）蛋白是 JA 信号途径中的转录抑制子。过量表达 JAZ7 被发现可赋予拟南芥干旱耐性，但具体的分子机制尚不清楚。本研究采用串联质量标签（TMT）定量蛋白质组学和靶向代谢组学方法，发现干旱及/或在三个基因型（野生型（WT）、JAZ7 敲除（KO）和 JAZ7 过表达（OE））之间有 394 种独特蛋白和 96 种代谢物差异表达。分析每种比较后的特有和差异蛋白/代谢物，以评估其在干旱耐性中的潜在功能。这些蛋白和代谢物在 JA 和脱落酸（ABA）信号通路、应激响应、光合作用、氧化还原和代谢过程中富集。研究意义：干旱胁迫是影响农业生产的全球性挑战。JAZ7 过表达通过调节光合作用、氧化还原和氨基酸、植物激素以及防御代谢物，赋予拟南芥干旱耐性。该研究结果为 JAZ7 调控的干旱耐性分子网络提供了重要见解。该研究结果可能有助于在气候变化时代提高作物的干旱耐性。
