MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Biosens Bioelectron. 2019 Apr 1;130:55-64. doi: 10.1016/j.bios.2019.01.027. Epub 2019 Jan 21.
In this work, a novel facile closed bipolar electrochemiluminescence (C-BP-ECL) sensor has been developed for highly sensitive detection of glucose based on the integration of chitosan (CS), poly(diallyldimethylammonium chloride)-functioned multi-walled carbon nanotubes (PDDA-MWCNTs) and graphene quantum dots-gold nanoparticles (GQDs-AuNPs) on the wax/carbon ink-screen-printed cloth-based device. When CS, PDDA-MWCNTs and GQDs-AuNPs are successively decorated onto the cathode of closed bipolar electrode (C-BPE), the C-BPE anode can emit much stronger C-BP-ECL signals. Moreover, the cathodic decoration of the C-BPE can generate a stronger ECL signal in comparison with its anodic decoration. Under optimized conditions, glucose can be detected in the range of 0.1-5000 μM, and the limit of detection is estimated to be 64 nM, which is about three orders of magnitude lower than that in case of the bare C-BPE cathode (31 μM). It has been shown that the proposed sensor has high detection sensitivity, wide dynamic range, and as well acceptable reproducibility, selectivity and stability. Finally, the applicability and validity of the C-BP-ECL sensor are demonstrated for the detection of glucose in human serum samples. We believe that this novel highly-sensitive sensor will have potential applications in various areas such as clinical diagnosis, food analysis and environmental monitoring.
在这项工作中，基于壳聚糖（CS）、聚二烯丙基二甲基氯化铵功能化多壁碳纳米管（PDDA-MWCNTs）和石墨烯量子点-金纳米粒子（GQDs-AuNPs）在蜡/碳油墨丝网印刷布基器件上的集成，开发了一种新颖的简便双极电化学发光（C-BP-ECL）传感器，用于葡萄糖的高灵敏度检测。当 CS、PDDA-MWCNTs 和 GQDs-AuNPs 相继修饰到封闭双极电极（C-BPE）的阴极上时，C-BPE 的阳极可以发出更强的 C-BP-ECL 信号。此外，与阳极修饰相比，C-BPE 的阴极修饰可以产生更强的 ECL 信号。在优化条件下，葡萄糖可以在 0.1-5000 μM 的范围内检测到，检测限估计为 64 nM，比裸 C-BPE 阴极（31 μM）低三个数量级。结果表明，所提出的传感器具有高检测灵敏度、宽动态范围以及可接受的重现性、选择性和稳定性。最后，该 C-BP-ECL 传感器在人血清样品中葡萄糖的检测中表现出了适用性和有效性。我们相信，这种新型的高灵敏度传感器将在临床诊断、食品分析和环境监测等各个领域具有潜在的应用前景。
