琼脂平板计数的准确性和速度

Accuracy and Speed in Counting Agar Plates .

作者信息

Fruin John T, Idll Terrel M, Clarke James B, Fowler James L, Guthertz Linda S

机构信息

Letterman Army Institute of Research, Department of Nutrition, Food Hygiene Division, Presidio of San Francisco, California 94129.

出版信息

J Food Prot. 1977 Sep;40(9):596-599. doi: 10.4315/0362-028X-40.9.596.

DOI:10.4315/0362-028X-40.9.596

PMID:30731586

Abstract

To determine the accuracy of colony counts made by analysts, agar plates were photographed. The agar plates and photographs were compared to obtain a true count (photocount) which was used to determine analyst accuracy over selected count ranges. Analyst accuracy was also determined by comparing analyst's counts to the mean of the counts obtained by several analysts ("established standard"). The "established standard" compared favorably with the photocount. Analysts' counts were within 5% of the photocount and "established standard" on 60 and 68% of plates having 30-300 colonies and 60 and 67% on plates having 20-200 colonies, respectively. Average counting time for plates in the 10-100, 20-200, 30-300, and 40-400 colony count ranges was 18, 30, 41, and 52 sec, respectively. Plates having 20-200 colonies were as suitable for counting as plates having 30-300 colonies and were counted with a time-saving of 27%.

摘要

为了确定分析人员进行菌落计数的准确性，对琼脂平板进行了拍照。将琼脂平板和照片进行比较以获得真实计数（照片计数），该计数用于确定分析人员在选定计数范围内的准确性。分析人员的准确性还通过将其计数与几位分析人员获得的计数平均值（“既定标准”）进行比较来确定。“既定标准”与照片计数相比表现良好。在有30 - 300个菌落的平板中，分析人员的计数分别在照片计数的5%以内，在有20 - 200个菌落的平板中，分别在照片计数和“既定标准”的60%和67%以内。在菌落计数范围为10 - 100、20 - 200、30 - 300和40 - 400的平板中，平均计数时间分别为18秒、30秒、41秒和52秒。有20 - 200个菌落的平板与有30 - 300个菌落的平板一样适合计数，且计数时间节省了27%。