Perry K L, McLane H
Cornell University, Department of Plant Pathology and Plant-Microbe Interactions, Ithaca, NY 14853.
Plant Dis. 2011 May;95(5):619. doi: 10.1094/PDIS-10-10-0768.
Potato virus M (PVM) was detected in upstate New York in two plants of the widely naturalized, weedy perennial Solanum dulcamara. The virus was detected with a macroarray assay for potato viruses (1). Amplified, complimentary DNAs from the two isolates hybridized to 5 and 7 of the 15 oligonucleotide probes for PVM. Testing of the samples by double-antibody sandwich-ELISA using PVM-specific antibodies (Agdia, Elkhart, IN) showed a clear positive result. Sequence information for a 118-bp genomic region was obtained by amplification using carlavirus-specific primers (2) (GenBank Accession No. HQ446853). Comparison with a reference PVM genome (GenBank Accession No. NC_001361) showed that the sequence corresponded to nucleotide positions 8418 to 8533 with 86% identity. The infected plants were symptomless and collected from two sites, 50 miles apart. One site was a weedy roadside location in Tompkins County in 2009, while the second was from a hedgerow in a (non-potato) vegetable production area of Ontario County in 2010. The virus could be detected throughout the growing season in this perennial host. PVM was reported from S. dulcamara L. in Hungary and described as being found frequently from a diversity of habitats (3). Importantly, the virus was transmitted via tubers and by Myzus persicae with low efficiency (3). These results suggest that the virus may be endemic in S. dulcamara in the northeastern United States and this host may serve as a reservoir for the virus from which it could move into potato. To our knowledge, PVM has not been reported in this host in North America. References: (1) B. Agindotan and K. L. Perry. Plant Dis. 92:730, 2008. (2) J. Badge et al. Eur. J. Plant Pathol. 102:305, 1996. (3) P. Salamon. Eur. Assoc. Pot. Res. Virol. Sect. Meet. 42:121, 2006.
在纽约州北部的两株广泛归化的多年生杂草龙葵中检测到马铃薯M病毒（PVM）。使用针对马铃薯病毒的宏阵列检测法（1）检测到了该病毒。来自两个分离株的扩增互补DNA与15个PVM寡核苷酸探针中的5个和7个杂交。使用PVM特异性抗体（Agdia，美国印第安纳州埃尔克哈特）通过双抗体夹心ELISA对样品进行检测，结果显示为明显阳性。使用香石竹潜隐病毒属特异性引物（2）扩增获得了一个118bp基因组区域的序列信息（GenBank登录号HQ446853）。与参考PVM基因组（GenBank登录号NC_001361）比较表明，该序列对应于核苷酸位置8418至8533，同一性为86%。受感染的植株无症状，分别采自相距50英里的两个地点。一个地点是2009年汤普金斯县杂草丛生的路边，另一个地点是2010年安大略县（非马铃薯）蔬菜产区的树篱。在这个多年生寄主的整个生长季节都能检测到该病毒。匈牙利曾报道过龙葵中的PVM，并描述其在多种生境中频繁出现（3）。重要的是，该病毒通过块茎和桃蚜传播，效率较低（3）。这些结果表明，该病毒可能在美国东北部的龙葵中呈地方性流行，并且这个寄主可能作为该病毒的储存库，病毒可由此传播到马铃薯中。据我们所知，北美尚未报道过该寄主中的PVM。参考文献：（1）B. Agindotan和K. L. Perry。植物病害。92:730，2（2）J. Badge等人。欧洲植物病理学杂志。102:（3）P. Salamon。欧洲马铃薯研究病毒学分会会议。42:121，2006。 08年。 305，1996年。
