Quintaneiro Carla, Soares Amadeu M V M, Costa Diana, Monteiro Marta S
a Department of Biology & CESAM , University of Aveiro , Aveiro , Portugal.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2019;54(5):478-483. doi: 10.1080/10934529.2019.1568793. Epub 2019 Feb 7.
Polychlorinated biphenyls (PCBs) are a class of industrial chemicals that cause endocrine changes, since they are able to bind to estrogen receptors and interfere with estrogen-regulated processes, such as fish vitellogenesis. Therefore, the present work aimed to assess potential endocrine effects of PCB-77 exposure during zebrafish (Danio rerio) gonadal differentiation. To achieve that, zebrafish juveniles were exposed to increasing concentrations of PCB-77 for 14 days during a critical window of gonad differentiation (30-44 days post-fertilization). Vitellogenin (Vtg) levels and several endpoints such as survival, growth, gonadosomatic index (GSI) and hepatosomatic index were recorded at the end of exposure and/or after 3 months in clean medium. The results obtained showed a reduction of Vtg-like proteins in juveniles, just after exposure to PCB-77 accompanied, after 3 months, by a decrease in gonadal Vtg levels and GSI of females. These results suggest that exposure to PCB-77 during the critical window of gonadal differentiation decreased vitellogenesis in juvenile zebrafish which lasted until adulthood affecting the normal development of female gonad, which might have further implications in reproduction success.
多氯联苯（PCBs）是一类工业化学品，会引起内分泌变化，因为它们能够与雌激素受体结合并干扰雌激素调节的过程，如鱼类卵黄生成。因此，本研究旨在评估斑马鱼（Danio rerio）性腺分化期间暴露于多氯联苯-77的潜在内分泌影响。为实现这一目标，在性腺分化的关键时期（受精后30-44天），将斑马鱼幼鱼暴露于浓度不断增加的多氯联苯-77中14天。在暴露结束时和/或在清洁培养基中培养3个月后，记录卵黄蛋白原（Vtg）水平以及几个指标，如存活率、生长率、性腺体指数（GSI）和肝体指数。所获得的结果表明，幼鱼在暴露于多氯联苯-77后，Vtg样蛋白减少，3个月后，雌性性腺Vtg水平和GSI下降。这些结果表明，在性腺分化的关键时期暴露于多氯联苯-77会降低斑马鱼幼鱼的卵黄生成，这种影响一直持续到成年期，影响雌性性腺的正常发育，这可能对繁殖成功产生进一步影响。
