非酯化脂肪酸和β-羟基丁酸对临床酮病奶牛肝脏CYP2E1的影响。
The effects of non-esterified fatty acids and β-hydroxybutyrate on the hepatic CYP2E1 in cows with clinical ketosis.
作者信息
Peng Zhicheng, Li Xiaobing, Wang Zhe, Liu Guowen, Li Xinwei
机构信息
Key Laboratory of Zoonosis, Ministry of Education,College of Veterinary Medicine, Jilin University,5333 Xi'an Road, Changchun, 130062, Jilin,China.
出版信息
J Dairy Res. 2019 Feb;86(1):68-72. doi: 10.1017/S0022029919000025. Epub 2019 Feb 8.
Dairy cows with ketosis display severe oxidative stress as well as high blood concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB). Cytochrome P4502E1 (CYP2E1) plays an important role in the induction of oxidative stress. The aim of this study was to investigate CYP2E1 expression and activity in the liver of clinically ketotic cows (in vivo) and the effects of NEFA and BHB on CYP2E1 expression and activity in hepatocytes (in vitro). Dairy cows with clinical ketosis exhibited a low blood concentration of glucose but high concentrations of NEFA and BHB. Hepatic mRNA, protein expression, and activity of CYP2E1 were significantly higher in cows with clinical ketosis than in control cows. In vitro, both NEFA and BHB treatment markedly up-regulated the mRNA and protein expressions as well as activity of CYP2E1 in cow hepatocytes. Taken together, these results indicate that high levels of NEFA and BHB significantly up-regulate the expression and activity of hepatic CYP2E1, and may be influential in the induction of oxidative stress in cows with clinical ketosis.
患有酮病的奶牛表现出严重的氧化应激以及血液中非酯化脂肪酸（NEFA）和β-羟基丁酸（BHB）的高浓度。细胞色素P4502E1（CYP2E1）在氧化应激的诱导中起重要作用。本研究的目的是调查临床酮病奶牛肝脏中CYP2E1的表达和活性（体内）以及NEFA和BHB对肝细胞中CYP2E1表达和活性的影响（体外）。患有临床酮病的奶牛血糖浓度低，但NEFA和BHB浓度高。临床酮病奶牛肝脏中CYP2E1的mRNA、蛋白质表达和活性显著高于对照奶牛。在体外，NEFA和BHB处理均显著上调了奶牛肝细胞中CYP2E1的mRNA和蛋白质表达以及活性。综上所述，这些结果表明高水平的NEFA和BHB显著上调肝脏CYP2E1的表达和活性，并且可能对临床酮病奶牛氧化应激的诱导有影响。
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