Pedram Majid
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
J Nematol. 2017 Mar;49(1):21-26. doi: 10.21307/jofnem-2017-042.
n. sp., a new species of the rare genus is described and illustrated based on its morphological and molecular characters. It was recovered from southern Iran. Females of the new species are characterized by having 1,511.3- to 1,792.5-µm long slender body, angular lip region having prominent papillae, 12- to 13-µm long odontostyle, double guiding ring, simple rod-like odontophore, didelphic-amphidelphic reproductive system, and 102- to 160-µm long elongate-conoid tail with rounded tip. Males of the new species are abundant and functional, characterized by 1,484- to 1,576-µm long body, 40- to 43-µm long spicules, 5 to 6 ventromedian supplements, and ventrally bent elongate conical tail. Compared to the type species, , the new species has differences in its tail morphology and value. These morphological differences and the separation of two species was further supported with basic differences in sequences of 28S rDNA D2/D3 and internal transcribed spacer 1 (ITS1) fragments. Compared to , it has basic differences in tail characters and spicule lengths. Molecular phylogenetic studies using partial sequences of 28S rDNA D2/D3 fragment of the new species and available sequences of Nordiidae members and several other dorylaim species/genera, revealed n. sp. and forming a clade with 0.81 Bayesian posterior probability (BPP). This clade forms a sister clade to the clade of sp. and , again with 0.81 BPP. In ITS1 tree, reconstructed using few available sequences, the new species and formed a clade with 0.98 BPP.
新物种，一个稀有属的新物种，基于其形态学和分子特征进行了描述和图示。它是在伊朗南部发现的。新物种的雌性特征为身体细长，长1511.3至1792.5微米，唇区呈角状，有突出的乳突，齿针长12至13微米，双导环，简单的棒状齿托，双子宫 - 双端生殖系统，以及尾部长102至160微米，呈细长锥形且末端圆形。新物种的雄性数量丰富且具有功能，特征为身体长1484至1576微米，交合刺长40至43微米，有5至6个腹中线副附器，以及腹侧弯曲的细长锥形尾巴。与模式种相比，新物种在尾部形态和 值上存在差异。28S rDNA D2/D3和内转录间隔区1（ITS1）片段序列的基本差异进一步支持了这些形态学差异以及两个物种的区分。与相比，它在尾部特征和交合刺长度上存在基本差异。使用新物种的28S rDNA D2/D3片段部分序列以及诺氏线虫科成员和其他几个矛线目物种/属的可用序列进行的分子系统发育研究表明，新物种与形成一个分支，贝叶斯后验概率（BPP）为0.81。这个分支与和的分支形成姐妹分支，BPP同样为0.81。在使用少量可用序列重建的ITS1树中，新物种和形成一个分支，BPP为0.98。
