Othman Othman E, Khodary Muhammad G, El-Deeb Ayman H, Hussein Hussein A
Cell Biology Department, National Research Centre, Dokki, Egypt.
Virology Department, Veterinary Medicine College, Cairo University, Egypt.
J Genet Eng Biotechnol. 2018 Dec;16(2):513-518. doi: 10.1016/j.jgeb.2018.02.009. Epub 2018 Feb 28.
Foot and Mouth disease (FMD) is a contagious disease leads to economically loss in livestock production all over the world. This serious disease is caused due to the infection of the animal with a single-stranded RNA virus (FMDV). This study aimed to investigate the genetic polymorphism of BoLA-DRB3 gene in Egyptian buffalo as a candidate genetic marker included in multi-factorial process of FMD resistance/susceptibility. Also this work aimed to genetically characterization and serotyping of circulating FMD virus in Egypt during 2016. For serotyping of FMDV, RT-PCR was used for FMDV-positive samples and the results declared the presence of serotype O in all tested animals. The sequence analysis of FMDV samples revealed five different patterns for the detected serotype O which were submitted to GenBank under the accession Nos.: MG017361-MG017365. The 302-bp amplified fragments from BoLA-DRB3 exon 2 were digested with III endonuclease and the results showed that the presence of five BoLA-DRB3 genotypes, among them the genotype AA might be associated with FMD-resistance (P < 0.01). On the other hand, genotype AC could be correlated with susceptibility (P < 0.01) to FMD in Egyptian buffaloes where it was absent in resistant group. The five detected genotypes of BoLA-DRB3 exon 2 were submitted to GenBank with the accession Nos.: MF977316-MF977320. In conclusion, our findings suggested that the detection of different BoLA-DRB3 genotypes may be has a promising role for raising the resistance of Egyptian buffalo against FMDV especially serotype O which is prevalent in Egypt with preferring genotype AA.
口蹄疫（FMD）是一种传染性疾病，会给全球牲畜生产带来经济损失。这种严重疾病是由动物感染单链RNA病毒（FMDV）引起的。本研究旨在调查埃及水牛中BoLA-DRB3基因的遗传多态性，将其作为口蹄疫抗性/易感性多因素过程中的候选遗传标记。此外，这项工作还旨在对2016年埃及流行的口蹄疫病毒进行基因特征分析和血清型鉴定。对于口蹄疫病毒的血清型鉴定，对FMDV阳性样本使用了RT-PCR，结果表明在所有测试动物中均存在O型血清型。口蹄疫病毒样本的序列分析揭示了检测到的O型血清型的五种不同模式，这些模式已提交至GenBank，登录号为：MG017361 - MG017365。用III型内切酶消化来自BoLA-DRB3外显子2的302 bp扩增片段，结果显示存在五种BoLA-DRB3基因型，其中AA基因型可能与口蹄疫抗性相关（P < 0.01）。另一方面，AC基因型可能与埃及水牛对口蹄疫的易感性相关（P < 0.01），抗性组中不存在该基因型。BoLA-DRB3外显子2检测到的五种基因型已提交至GenBank，登录号为：MF977316 - MF977320。总之，我们的研究结果表明，检测不同的BoLA-DRB3基因型可能在提高埃及水牛对口蹄疫病毒的抗性方面具有重要作用，特别是对在埃及流行的O型血清型，其中AA基因型更受青睐。
