An Tian, He Zhong-Chen, Zhang Xin-Qing, Li Jun, Chen Ai-Ling, Tan Fang, Chen Hong-Dong, Lv Bo-Han, Lian Juan, Gao Si-Hua, Jiang Guang-Jian
1Diabetes Research Center, Traditional Chinese Medicine School, Beijing University of Chinese Medicine, Beijing, 100029 China.
Department of endocrinology, Beijing He ping li Hospital, Beijing, 100013 China.
Chin Med. 2019 Feb 1;14:3. doi: 10.1186/s13020-019-0225-1. eCollection 2019.
Baduanjin, a traditional Chinese exercise therapy, has been widely used in China to treat type 2 diabetes (T2DM) with depression (DD). However, the underlying mechanism of Baduanjin in anti-DD is unclear. This study was focused on investigating the effects of Baduanjin on symptoms of depression and blood glucose in patients with DD and the underlying mechanism.
We performed a 12-week Baduanjin intervention on patients with DD and longitudinally compared the differential expressions of lncRNAs, circRNAs, and mRNAs between pre- (BDD) and post- (ADD) Baduanjin intervention in the same group. Subsequently, Gene Ontology (GO) and pathway analysis was performed to investigate the function of differentially expressed mRNAs. Finally, Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to verify the sequencing result and the mRNA-lncRNA regulatory network was constructed.
The blood glucose level, depression index scores, and PHQ9 scores of the patients with DD were significantly decreased (P < 0.05) after Baduanjin intervention. Compared to BDD, 207 lncRNAs, 266 circRNAs, and 610 differentially expressed mRNAs were identified in ADD. Kyoto Encyclopedia of Genes and Genomes (KEGG) and GO showed that the significantly dysregulated mRNAs were mainly involved in immune function and inflammatory response pathways, and various signaling pathways including IL-17 and TNF. In addition, we selected five differentially expressed lncRNAs to construct an lncRNA-mRNA regulatory network, and found a total of 1045 mRNAs associated with them.
Our research is the first systematic profiling of mRNA, lncRNA, and circRNA in patients of ADD and BDD, and provides valuable insights in the potential mechanism of Baduanjin in anti-DD. Further, it was confirmed that Baduanjin is a safe and effective intervention for patients with DD because it can effectively ameliorate the symptoms of depression and blood glucose levels in patients with DD by regulating the dysregulated expression of lncRNA, mRNA, and circRNA.
八段锦作为一种传统的中医疗法，在中国已被广泛用于治疗伴有抑郁的2型糖尿病（T2DM）。然而，八段锦抗抑郁的潜在机制尚不清楚。本研究旨在探讨八段锦对伴有抑郁的患者抑郁症状和血糖的影响及其潜在机制。
我们对伴有抑郁的患者进行了为期12周的八段锦干预，并纵向比较了同一组患者在八段锦干预前（BDD）和干预后（ADD）lncRNAs、circRNAs和mRNAs的差异表达。随后，进行基因本体论（GO）和通路分析以研究差异表达mRNA的功能。最后，使用逆转录-聚合酶链反应（RT-PCR）验证测序结果并构建mRNA-lncRNA调控网络。
八段锦干预后，伴有抑郁的患者的血糖水平、抑郁指数评分和PHQ9评分显著降低（P < 0.05）。与BDD相比，在ADD中鉴定出207个lncRNAs、266个circRNAs和610个差异表达的mRNAs。京都基因与基因组百科全书（KEGG）和GO显示，显著失调的mRNAs主要参与免疫功能和炎症反应通路，以及包括IL-17和TNF在内的各种信号通路。此外，我们选择了五个差异表达的lncRNAs构建lncRNA-mRNA调控网络，共发现1045个与之相关的mRNAs。
我们的研究首次对ADD和BDD患者的mRNA、lncRNA和circRNA进行了系统分析，并为八段锦抗抑郁的潜在机制提供了有价值的见解。此外，证实八段锦对伴有抑郁的患者是一种安全有效的干预措施，因为它可以通过调节lncRNA、mRNA和circRNA的失调表达有效改善伴有抑郁的患者的抑郁症状和血糖水平。
