Departments of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical College, No.287 Changhuai Road, Bengbu City, Anhui Province, 233004, People's Republic of China.
Department of Respiratory Diseases, The First Affiliated Hospital of Bengbu Medical College, No.287 Changhuai Road, Bengbu City, Anhui Province, 233004, People's Republic of China.
Invest New Drugs. 2019 Dec;37(6):1127-1134. doi: 10.1007/s10637-018-00721-z. Epub 2019 Feb 8.
Long non-coding RNAs (lncRNAs) are increasingly recognized as important regulators in tumor development. This study aims to investigate the potential role oflncRNALEF1-AS1, in the progression of lung cancer. Quantitative real-time PCR (qRT-PCR) and western blot assays showed that LEF1-AS1 was upregulated while miR-544a was downregulated in lung cancer specimens and cells. Overexpression of LEF1-AS1 led to the enhancement of cell proliferation and invasion, revealed by CCK-8 assay and transwell assay. A negative correlation was found between LEF1-AS1 and miR-544a. BLAST analysis and dual-luciferase assay confirmed that FOXP1 is a downstream effector of miR-544a. Therefore, the LEF1-AS1/miR-544a/FOXP1 axis is an important contributor to lung cancer progression. Collectively, our novel data uncovers a new mechanism that governs tumor progression in lung cancer and provides new targets that may be used for disease monitoring and therapeutic intervention of lung cancer.
长链非编码 RNA（lncRNAs）在肿瘤发展中被越来越多地认为是重要的调控因子。本研究旨在探讨 lncRNA LEF1-AS1 在肺癌进展中的潜在作用。定量实时 PCR（qRT-PCR）和 Western blot 检测显示，LEF1-AS1 在肺癌标本和细胞中上调，而 miR-544a 下调。CCK-8 检测和 Transwell 检测显示，LEF1-AS1 的过表达导致细胞增殖和侵袭增强。LEF1-AS1 与 miR-544a 之间存在负相关。BLAST 分析和双荧光素酶报告基因检测证实 FOXP1 是 miR-544a 的下游效应因子。因此，LEF1-AS1/miR-544a/FOXP1 轴是肺癌进展的重要贡献者。总之，我们的新数据揭示了一种新的机制，该机制调控了肺癌中的肿瘤进展，并提供了可能用于肺癌疾病监测和治疗干预的新靶点。
