Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, China.
Luminescence. 2019 May;34(3):334-340. doi: 10.1002/bio.3611. Epub 2019 Feb 7.
We present an innovative and sensitive electrogenerated chemiluminescence (ECL) strategy for observing the surface feature of a single silica nanoparticle based on its collision with an ultramicroelectrode (UME). As an ECL luminophore, Ru(bpy) molecules are doped into silica nanoparticles. The stochastic collision events of Ru(bpy) -doped silica nanoparticles (RuSNPs) can be tracked by observing the ECL 'blips' from the ECL reaction of Ru(bpy) with a coreactant in solution. When RuSNPs collided with UME, Ru(bpy) molecules that only exist near the collision site of silica nanoparticles (NPs) were electrochemically oxidized to form Ru(bpy) , and then emitted light, because silica NPs are insulated. The inhomogeneous properties of silica nanoparticle surfaces will produce diverse ECL blips in intensity and shape. In addition, distribution gradients from the he Ru(bpy) in a silica matrix also affect ECL blips. Some information on the surface properties of silica NPs can be obtained by observation of single silica collision events.
我们提出了一种基于胶态纳米颗粒与超微电极碰撞的新颖而灵敏的电致化学发光（ECL）策略，用于观察单个二氧化硅纳米颗粒的表面特征。以 Ru(bpy) 分子作为 ECL 发光体，掺杂到二氧化硅纳米颗粒中。通过观察溶液中 Ru(bpy) 与共反应物的 ECL 反应产生的 ECL“闪烁”，可以跟踪 Ru(bpy) 掺杂的二氧化硅纳米颗粒（RuSNPs）的随机碰撞事件。当 RuSNPs 与 UME 碰撞时，仅存在于二氧化硅纳米颗粒（NPs）碰撞位点附近的 Ru(bpy) 分子被电化学氧化形成 Ru(bpy) ，并发出光，因为二氧化硅 NPs 是绝缘的。二氧化硅纳米颗粒表面的不均匀性质会导致强度和形状各异的 ECL 闪烁。此外，二氧化硅基质中 Ru(bpy) 的分布梯度也会影响 ECL 闪烁。通过观察单个二氧化硅碰撞事件，可以获得有关二氧化硅纳米颗粒表面性质的一些信息。
