a Department of Intervention Therapy , Second Affiliated Hospital of Dalian Medical University , Dalian , Liaoning , China.
b Department of Medical Oncology , Dalian Fifth People's Hospital , Dalian , Liaoning , China.
J Enzyme Inhib Med Chem. 2019 Dec;34(1):420-428. doi: 10.1080/14756366.2018.1484734.
This study aimed to identify genetic biomarkers in pancreatic cancer (PC) and explore its function in PC via a feature-base analysis of bioinformatics. OMIM and DisGeNET databases discovered 209 PC connected genes and then 516 connected genes were identified. We selected 29 genes according to optimal features and chose COL1A2, which had the highest expression, for the following experiment. The expression of COL1A2 was determined by qRT-PCR; cell proliferation was determined by MTT assay; migration and invasion after COL1A2 and miR-25-3p transfection was evaluated by Transwell assay. COL1A2 presented the highest expression in PC tissues, which was validated in functional experiments. MiR-25-3p suppressed the expression of COL1A2 in cell lines and inhibited migration, invasion and proliferation of PC cells. MiR-25-3p could suppress the expression of COL1A2 and inhibit the proliferation, migration and invasion of PC cells which provided a new idea for the detection and treatment of PC.
本研究旨在通过生物信息学的特征基础分析鉴定胰腺癌（PC）中的遗传生物标志物，并探讨其在 PC 中的功能。通过 OMIM 和 DisGeNET 数据库发现了 209 个与 PC 相关的基因，然后又鉴定出了 516 个相关基因。我们根据最佳特征选择了 29 个基因，并选择表达量最高的 COL1A2 进行后续实验。通过 qRT-PCR 确定 COL1A2 的表达，通过 MTT 测定细胞增殖，通过 Transwell 测定 COL1A2 和 miR-25-3p 转染后的迁移和侵袭。COL1A2 在 PC 组织中表达最高，在功能实验中得到了验证。miR-25-3p 抑制细胞系中 COL1A2 的表达，并抑制 PC 细胞的迁移、侵袭和增殖。miR-25-3p 可以抑制 COL1A2 的表达，抑制 PC 细胞的增殖、迁移和侵袭，为 PC 的检测和治疗提供了新的思路。
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