Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College of Shihezi University, Xinjiang, China.
Eur Rev Med Pharmacol Sci. 2017 Sep;21(18):4050-4057.
Pancreatic cancer (PC) is the most malignant tumor among all the tumors in the digestive system. MiR-217 has been reported to take a critical part in various malignant tumors. The aim of this study was to explore the function of MiR-217 in pancreatic cancer and its target genes.
Twenty pairs of PC tissues and matched normal adjacent pancreatic tissues were collected. The expression of miR-217 in PC tissues and normal pancreatic tissues was detected by Real-time polymerase chain reaction (PCR). PC cells were transfected with miR-217 mimics, inhibitors and negative control, respectively. Cell Counting Kit-8 (CCK-8) assay was used to detect cell viability. Cell apoptosis was checked via Annexin V-FITC/PI apoptosis kit. The protein expression of E2F3 was detected by Western blot. To detect repression by miR-217, HEK293T cells were co-transfected with the indicated E2F3 3'-UTR luciferase reporter.
The expression of miR-217 was reduced in PC tissues comparing to normal pancreatic tissues. Meantime, the in-vitro study revealed that miR-217 suppressed PC cell growth, invasion but promoted apoptosis. Next, we proved that E2F3 was the target of miR-217 on PC cell function.
miR-217 suppresses PC cell growth, invasion but promotes apoptosis in vitro through targeting E2F3. The miR-217-E2F3 axis may be used for PC therapy.
胰腺癌（PC）是消化系统所有肿瘤中最恶性的肿瘤。已经有报道称 miR-217 在各种恶性肿瘤中起着关键作用。本研究旨在探讨 miR-217 在胰腺癌中的功能及其靶基因。
收集 20 对 PC 组织和匹配的正常胰腺相邻组织。通过实时聚合酶链反应（PCR）检测 miR-217 在 PC 组织和正常胰腺组织中的表达。分别用 miR-217 模拟物、抑制剂和阴性对照转染 PC 细胞。细胞计数试剂盒-8（CCK-8）检测细胞活力。通过 Annexin V-FITC/PI 凋亡试剂盒检测细胞凋亡。用 Western blot 检测 E2F3 的蛋白表达。为检测 miR-217 的抑制作用，将指示的 E2F3 3'-UTR 荧光素酶报告基因共转染至 HEK293T 细胞。
与正常胰腺组织相比，miR-217 在 PC 组织中的表达降低。同时，体外研究表明 miR-217 抑制 PC 细胞生长、侵袭，但促进细胞凋亡。接下来，我们证明 E2F3 是 miR-217 对 PC 细胞功能的靶基因。
miR-217 通过靶向 E2F3 抑制 PC 细胞的生长、侵袭，促进体外细胞凋亡。miR-217-E2F3 轴可能用于 PC 治疗。
