Passineau Michael J, Gallo Phillip H, Williams Gretchen, Perez Reina, Benza Raymond L
Cardiovascular Institute, Allegheny Health Network;
Cardiovascular Institute, Allegheny Health Network.
J Vis Exp. 2019 Jan 23(143). doi: 10.3791/58353.
A variety of pathologies lead to pulmonary hypertension (PH), which is defined as a mean pulmonary artery pressure exceeding 25 mmHg at rest. To further diagnose and manage PH, patients undergo repeated right heart catheterizations (RHC) wherein a Swan-Ganz catheter is advanced into a branch of the pulmonary artery and a balloon is inflated to wedge the catheter tip. This article illustrates a protocol whereby pulmonary artery endothelial cells (PAECs) may be harvested from the balloon tips of Swan-Ganz catheters after RHC, and purified with an anti- CD146 affinity column technique to purify putative PAECs. These cells might be used to provide an in situ snapshot of the biological state of the pulmonary vasculature endothelium to complement hemodynamic measurements obtained during RHC. Harvested and purified PAECs may be used for either cell culture or for subsequent analytical assays such as flow cytometery.
多种病理状况可导致肺动脉高压（PH），其定义为静息时平均肺动脉压超过25 mmHg。为了进一步诊断和管理肺动脉高压，患者需接受多次右心导管插入术（RHC），即将Swan-Ganz导管推进到肺动脉分支中，并使球囊膨胀以楔入导管尖端。本文阐述了一种方案，通过该方案可在右心导管插入术后从Swan-Ganz导管的球囊尖端采集肺动脉内皮细胞（PAECs），并用抗CD146亲和柱技术进行纯化，以纯化假定的PAECs。这些细胞可用于提供肺血管内皮生物状态的原位快照，以补充右心导管插入术期间获得的血流动力学测量结果。采集和纯化的PAECs可用于细胞培养或后续分析检测，如流式细胞术。
