Asad Sultan, Feitosa-Suntheimer Fabiana, Gold Alexander, Londono-Renteria Berlin, Colpitts Tonya M
Department of Microbiology, Boston University School of Medicine; National Emerging Infectious Diseases Laborator, Boston University School of Medicine.
National Emerging Infectious Diseases Laborator, Boston University School of Medicine.
J Vis Exp. 2019 Jan 18(143). doi: 10.3791/58691.
The recent emergence of the flavivirus Zika and neurological complications, such as Guillain-Barré syndrome and microcephaly in infants, has brought serious public safety concerns. Among the risk factors, antibody-dependent enhancement (ADE) poses the most significant threat, as the recent re-emergence of the Zika virus (ZIKV) is primarily in areas where the population has been exposed and is in a state of pre-immunity to other closely related flaviviruses, especially dengue virus (DENV). Here, we describe a protocol for quantifying the effect of human serum antibodies against DENV on ZIKV infection in primary human cells or cell lines.
黄病毒寨卡病毒的近期出现以及诸如格林-巴利综合征和婴儿小头畸形等神经并发症引发了严重的公共安全担忧。在风险因素中，抗体依赖性增强（ADE）构成了最重大的威胁，因为寨卡病毒（ZIKV）近期的再度出现主要是在人群已接触过且对其他密切相关黄病毒（尤其是登革病毒，DENV）处于预免疫状态的地区。在此，我们描述了一种用于量化人血清中抗登革病毒抗体对原代人细胞或细胞系中寨卡病毒感染影响的实验方案。
