O'Rourke Matthew B, Smith Caine C, De La Monte Suzanne M, Sutherland Greg T, Padula Matthew P
Sydney Mass Spectrometry, Charles Perkins Centre, The University of Sydney, Camperdown, New South Wales, Australia.
Proteomics Core Facility, University of Technology Sydney, Ultimo, New South Wales, Australia.
Curr Protoc Mol Biol. 2019 Apr;126(1):e86. doi: 10.1002/cpmb.86. Epub 2019 Feb 8.
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a well-established technique for elucidating the location and relative abundance of a range of biomolecules. More recently, research into this technique has shifted from simple discovery and demonstration of utility to application in biomedical research. Here, we describe a protocol utilizing MALDI-IMS for the spatial mapping of lipids in brain tissue from normal human brains and brains from patients with Alzheimer's disease, in the context of Alzheimer's disease. Improved accuracy calibration of the instrument from the tissue surface is emphasized, as this allows for significantly improved mass determination in time of flight (TOF)-based instruments enabling more confident preliminary lipid identification. This improved initial result allows MALDI-IMS data to be complemented with additional instrumentation, such as liquid chromatography mass spectrometry workflows or specialized non-TOF systems such as Fourier transform cyclotron resonance instruments. This method is not limited to human tissue and can be applied to virtually any lipid-rich formalin-fixed tissue. © 2019 by John Wiley & Sons, Inc.
基质辅助激光解吸/电离成像质谱（MALDI-IMS）是一种成熟的技术，用于阐明一系列生物分子的位置和相对丰度。最近，对该技术的研究已从简单的发现和实用性证明转向生物医学研究中的应用。在此，我们描述了一种在阿尔茨海默病背景下，利用MALDI-IMS对正常人脑和阿尔茨海默病患者脑组织中的脂质进行空间映射的方案。强调了从组织表面对仪器进行改进的精度校准，因为这可以显著提高基于飞行时间（TOF）的仪器的质量测定，从而使脂质的初步鉴定更具信心。这一改进的初始结果使得MALDI-IMS数据能够与其他仪器，如液相色谱质谱工作流程或专门的非TOF系统（如傅里叶变换回旋共振仪器）相结合。该方法不限于人体组织，几乎可应用于任何富含脂质的福尔马林固定组织。©2019约翰威立父子公司版权所有
