Moreton E, Baron P, Tiplady S, McCall S, Clifford B, Langley-Evans S C, Fone K C F, Voigt J P
School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, UK.
UCLA, MRL 3230/3240, 675 Charles E Young Drive South, Los Angeles, CA, 90095, United States.
Behav Brain Res. 2019 May 2;363:191-198. doi: 10.1016/j.bbr.2019.02.003. Epub 2019 Feb 5.
The prefrontal cortex (PFC) undergoes protracted postnatal development such that its structure and behavioural function may be profoundly altered by environmental factors. Here we investigate the effect of lactational dietary manipulations on novel object recognition (NOR) learning and PFC monoamine neurotransmitter metabolism in early adolescent rats. To this end, Wistar rat dams were fed a high caloric cafeteria diet (CD) during lactation and resultant 24-26 day old offspring exposed to NOR testing and simultaneous PFC dopamine and serotonin metabolism measurement. In the second NOR choice trial where one familiar and one novel object were presented controls explored the novel preferentially to the familiar object both after a 5 min (P < 0.001) or 30 min (P < 0.05) inter-trial intervals (ITI). By contrast, offspring from dams fed on lactational CD failed to show any significant preference for the novel object at either time point. Compared with chow fed controls, their average exploration ratio of the novel object was lower after the 5 min ITI (P < 0.05). Following a 60 min ITI, neither CD nor control offspring showed a preference for the novel object. PFC dopamine metabolism was significantly reduced in the CD group (P < 0.001), whereas serotonin metabolism was increased (P < 0.001). These results suggest that an obesogenic lactational diet can have a detrimental impact on cognition in adolescent offspring associated with aberrant PFC serotonin and dopamine metabolism.
前额叶皮质（PFC）在出生后经历长期发育，因此其结构和行为功能可能会受到环境因素的深刻影响。在此，我们研究哺乳期饮食操作对青春期早期大鼠新物体识别（NOR）学习及PFC单胺类神经递质代谢的影响。为此，在哺乳期给Wistar大鼠母鼠喂食高热量自助餐饮食（CD），并对由此产生的24 - 26日龄后代进行NOR测试，同时测量PFC多巴胺和血清素代谢。在第二次NOR选择试验中，呈现一个熟悉物体和一个新物体，在5分钟（P < 0.001）或30分钟（P < 0.05）的试验间隔（ITI）后，对照组对新物体的探索优先于熟悉物体。相比之下，哺乳期喂食CD的母鼠所产后代在两个时间点均未表现出对新物体的任何显著偏好。与喂食普通饲料的对照组相比，在5分钟ITI后，它们对新物体的平均探索率较低（P < 0.05）。在60分钟ITI后，CD组和对照组后代均未表现出对新物体的偏好。CD组的PFC多巴胺代谢显著降低（P < 0.001），而血清素代谢增加（P < 0.001）。这些结果表明，致肥胖的哺乳期饮食可能会对青春期后代的认知产生有害影响，这与PFC血清素和多巴胺代谢异常有关。
