Department of Chemistry, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Chemistry, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Int J Biol Macromol. 2019 May 15;129:84-90. doi: 10.1016/j.ijbiomac.2019.02.015. Epub 2019 Feb 5.
This work is directed towards the synthesis of the magnetic nanoparticle in a core and a gold shell with immobilized of laccase on its surface (FeO@Au@ Lac). The prepared FeO@Au@ Lac core/shell nanoparticles are characterized by means TEM, SEM, DLS, zeta potential, UV-Vis, and TGA. Meanwhile, as an example of the applications, Biosensor activity was investigated by using the oxidation of catechol and recording the UV-Vis absorption in the 402 nm wavelength. The biosensor also demonstrated optimum activity at pH 5.0, reaction time at 40 min, and 35 mg the amount of biosensor. Linear response in the catechol concentration range of 5.0-70.0 μM. The limit of detection and the apparent Michaels-Menten constant (K) of the biosensor were 2 μM and 15 μM respectively.
这项工作致力于合成具有固定化漆酶的磁性纳米粒子核-壳结构（FeO@Au@Lac）。所制备的 FeO@Au@Lac 核/壳纳米粒子通过 TEM、SEM、DLS、zeta 电位、UV-Vis 和 TGA 进行表征。同时，作为应用实例，通过氧化儿茶酚并记录 402nm 波长处的紫外-可见吸收来研究生物传感器的活性。该生物传感器在 pH 5.0、反应时间 40 分钟和 35mg 生物传感器的量下表现出最佳活性。在 5.0-70.0μM 的儿茶酚浓度范围内具有线性响应。生物传感器的检测限和表观米氏常数（K）分别为 2μM 和 15μM。
