Department of Chemistry and Chemical Engineering, Inha University, 100 Inharo, Namgu, Incheon, 22212, South Korea.
Department Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul, 139-706, South Korea.
Biomaterials. 2019 Apr;199:32-39. doi: 10.1016/j.biomaterials.2019.01.043. Epub 2019 Feb 2.
We introduce an efficient cell tracking imaging protocol using positron emission tomography (PET). Since macrophages are known to home and accumulate in tumor tissues and atherosclerotic plaque, we design a PET imaging protocol for macrophage cell tracking using aza-dibenzocyclooctyne-tethered PEGylated mesoporous silica nanoparticles (DBCO-MSNs) with the short half-life F-18-labeled azide-radiotracer via an in vivo strain-promoted alkyne azide cycloaddition (SPAAC) covalent labeling reaction inside macrophage cells in vivo. This PET imaging protocol for in vivo cell tracking successfully visualizes the migration of macrophage cells into the tumor site by the bioorthogonal SPAAC reaction of DBCO-MSNs with [F]fluoropentaethylene glycolic azide ([F]2) to form F-labeled aza-dibenzocycloocta-triazolic MSNs (F-DBCOT-MSNs) inside RAW 264.7 cells. The tissue radioactivity distribution results were consistent with PET imaging findings. In addition, PET images of atherosclerosis in ApoE mice fed a western diet for 30 weeks were obtained using the devised macrophage cell-tracking protocol.
我们介绍了一种使用正电子发射断层扫描（PET）进行高效细胞追踪成像的方案。由于已知巨噬细胞会归巢并积聚在肿瘤组织和动脉粥样硬化斑块中，因此我们设计了一种使用叠氮化物标记的放射性示踪剂通过体内应变促进的叠氮化物-炔烃环加成（SPAAC）共价标记反应标记带有短半衰期 F-18 标记的叠氮化物的偶氮二苯并环辛炔键合聚乙二醇化介孔硅纳米颗粒（DBCO-MSNs）进行巨噬细胞细胞追踪的 PET 成像方案，在体内巨噬细胞内。该用于体内细胞追踪的 PET 成像方案成功地通过 DBCO-MSN 与 [F]氟戊二酸叠氮化物（[F]2）的生物正交 SPAAC 反应，在 RAW 264.7 细胞内形成 F 标记的偶氮二苯并环辛三唑 MSNs（F-DBCOT-MSNs），从而可视化巨噬细胞向肿瘤部位的迁移。组织放射性分布结果与 PET 成像结果一致。此外，还使用设计的巨噬细胞细胞追踪方案获得了喂食西方饮食 30 周的 ApoE 小鼠动脉粥样硬化的 PET 图像。
