Bregitzer P, Dahleen L S, Campbell R D
United States Department of Agriculture, Agricultural Research Service, PO Box 307, Aberdeen, ID 83210 USA Fax: 208-397-4165 e-mail:
USDA-ARS, PO Box 5677, Fargo, ND 58105 USA, , , , , , US.
Plant Cell Rep. 1998 Sep;17(12):941-945. doi: 10.1007/s002990050514.
Genotypic restrictions on plant regeneration from cultured cells have hindered the genetic transformation of most barley cultivars. Optimizing culturing protocols for specific cultivars of commercial interest may facilitate their genetic transformation. Plant regeneration from embryogenic callus of Harrington', Morex', and `Hector' as affected by certain protocol modifications was examined in replicated experiments. Regeneration was improved for all cultivars by separately autoclaving certain components of the culture media and by reducing the amount of embryogenic callus cultured per petri dish. Regeneration improvements in response to various concentrations of copper and 2,4-dichlorophenoxyacetic acid were more genotype specific. This study suggests that the development and use of genotype-specific protocols can enhance plant regeneration. Enhancements in plant regeneration are expected to facilitate the transformation of commercial barley germplasm.
培养细胞再生植株的基因型限制阻碍了大多数大麦品种的遗传转化。优化具有商业价值的特定品种的培养方案可能会促进其遗传转化。在重复实验中，研究了某些方案修改对‘哈林顿’、‘莫雷克斯’和‘赫克托’胚性愈伤组织再生植株的影响。通过分别对培养基的某些成分进行高压灭菌以及减少每个培养皿中培养的胚性愈伤组织数量，所有品种的再生率均有所提高。对不同浓度的铜和2,4-二氯苯氧乙酸的再生反应改善更具基因型特异性。本研究表明，开发和使用基因型特异性方案可以提高植株再生率。预计植株再生率的提高将促进商业大麦种质的转化。
