Gless C, Lörz H, Jähne-Gärtner A
Institut für Allgemeine Botanik, AMP II, Ohnhorststraße 18, D-22609 Hamburg, Germany Fax: +49-40-82282-229 E-mail:
Plant Cell Rep. 1998 Apr;17(6-7):441-445. doi: 10.1007/s002990050422.
A reliable and efficient protocol for the regeneration of fertile plants derived from leaf base segments of young in-vitro-grown oat seedlings has been developed successfully. Callus induction and shoot regeneration were achieved when the basal region of young seedlings was cultured on auxin-containing medium. Callus induction efficiencies as well as regeneration frequencies were correlated with the developmental stage and the genotype of the explants. In five different genotypes of oat, an average of 25 plants per explant could be produced and for the most responsive genotype more than 50 regenerants per explant could be regenerated reproducibly. This high regeneration potential makes oat leaf bases a very attractive target for transformation.
已成功开发出一种可靠且高效的方案，用于从体外培养的燕麦幼苗叶基部再生可育植株。当将幼苗基部区域培养在含生长素的培养基上时，可实现愈伤组织诱导和芽再生。愈伤组织诱导效率和再生频率与外植体的发育阶段和基因型相关。在五种不同基因型的燕麦中，每个外植体平均可产生25株植株，对于反应最灵敏的基因型，每个外植体可重复再生50多个再生植株。这种高再生潜力使燕麦叶基部成为非常有吸引力的转化靶点。
